Catalog No: OKEH00637
Size:96T
Price: $425.00
SKU
OKEH00637
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Predicted Species Reactivity | Chicken, Gallus gallus | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology PRL ELISA Kit (Chicken) (OKEH00637) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Chicken PRL antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated PRL and incubated. The PRL found in the sample or standards competes with the biotinylated PRL for limited binding sites on the immobilized anti-Chicken PRL antibody. Excess unbound biotinylated PRL and sample or standard PRL is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated PRL captured in the well and inversely proportional to the amount of PRL which was contained in the sample or standard. | ||||||||||||||||||||||
ELISA Kit Range | 0.39-25ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 84% | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: < 5.3% (n = 20) Mean Inter-assay CV%: < 7.7% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids | ||||||||||||||||||||||
Sensitivity | 0.195 ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Chicken Prolactin | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Competitive ELISA |
Gene Symbol | PRL |
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Gene Full Name | prolactin |
Alias Symbols | prolactin;prolactin type 2a;prolactin type 2b;prolactin type 2c;prolactin type 3. |
NCBI Gene Id | 396453 |
Protein Name | P14676 |
Description of Target | Prolactin |
Uniprot ID | https://www.uniprot.org/uniprot/P14676 |
Protein Accession # | NP_990797.2 |
Nucleotide Accession # | NM_205466.2 |
Protein Size (# AA) | 229 |
Molecular Weight | 26 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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