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Catalog No: OKEH00637
Size:96T
Price: $425.00
SKU
OKEH00637
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH00637
Product Info
Predicted Species ReactivityChicken, Gallus gallus
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PRL ELISA Kit (Chicken) (OKEH00637) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Chicken PRL antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated PRL and incubated. The PRL found in the sample or standards competes with the biotinylated PRL for limited binding sites on the immobilized anti-Chicken PRL antibody. Excess unbound biotinylated PRL and sample or standard PRL is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated PRL captured in the well and inversely proportional to the amount of PRL which was contained in the sample or standard.
ELISA Kit Range0.39-25ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 84%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.3% (n = 20)
Mean Inter-assay CV%: < 7.7% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-PRL Microplate96 Wells (12 x 8 Well strips)
PRL Lyophilized Standard2
Sample Diluent1 x 20 mL
100X PRL-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.195 ng/mL
SpecificityNatural and recombinant Chicken Prolactin
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolPRL
Gene Full Nameprolactin
Alias Symbolsprolactin;prolactin type 2a;prolactin type 2b;prolactin type 2c;prolactin type 3.
NCBI Gene Id396453
Protein NameP14676
Description of TargetProlactin
Uniprot IDhttps://www.uniprot.org/uniprot/P14676
Protein Accession #NP_990797.2
Nucleotide Accession #NM_205466.2
Protein Size (# AA)229
Molecular Weight26 kDa
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