PRKDC ELISA Kit (Mouse) (OKCD01065)

Catalog No: OKCD01065 (Formerly GWB-KBBJP1)

Size:96WELLS

Mouse Prkdc ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Prkdc Microplate: 96 Wells (12 x 8 Well Strips)
  • Prkdc Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated Prkdc Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.105 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC). No significant cross-reactivity or interference between Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Prkdc
• Gene Full Name: protein kinase, DNA activated, catalytic polypeptide
• Alias Symbols: AI326420;AU019811;DNA;DNA-;DNA-dependent protein kinase catalytic subunit;DNAPDcs;DNAPK;DNA-PK catalytic subunit;DNA-PKcs;DNPK1;DOX;DOXNPH;doxorubicin nephropathy;dxn;dxnph;HYRC1;p460;scid;severe combined immunodeficiency;slip;XRCC;XRCC7.
• NCBI Gene Id: 19090
• Protein Name: DNA-dependent protein kinase catalytic subunit
• Description of Target: Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, C1D, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D. Contributes to the determination of the circadian period length by antagonizing phosphorylation of CRY1 'Ser-588' and increasing CRY1 protein stability, most likely through an indirect mechanism. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway (By similarity).
• Uniprot ID: P97313
• Protein Accession #: NP_035289
• Nucleotide Accession #: NM_011159.2
• Protein Size (# AA): 4128