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Datasheets/Manuals | Printable datasheet for PRKAG1 Antibody (OAAF02319) |
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Predicted Species Reactivity | Human|Mouse|Rat |
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Product Format | Liquid |
Clonality | Polyclonal |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Western blot |
Reconstitution and Storage | -20°C |
Immunogen | The antiserum was produced against synthesized peptide derived from human PRKAG1/2/3. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Specificity | PRKAG1/2/3 antibody detects endogenous levels of total PRKAG1/2/3 protein. |
Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Dilution | The optimal working dilution should be determined by the end user. |
Application Info | WB: 1:500~1:3000; ELISA: 1:5000 |
Reference | Gao G., J. Biol. Chem. 271:8675-8681(1996). The MGC Project Team, Genome Res. 14:2121-2127(2004). Baba M., Proc. Natl. Acad. Sci. U.S.A. 103:15552-15557(2006). |
Gene Symbol | PRKAG1|PRKAG2|PRKAG3 |
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Gene Full Name | protein kinase AMP-activated non-catalytic subunit gamma 1|protein kinase AMP-activated non-catalytic subunit gamma 2|protein kinase AMP-activated non-catalytic subunit gamma 3 |
Alias Symbols | 5'-AMP-activated protein kinase subunit gamma-1;5'-AMP-activated protein kinase subunit gamma-2;5'-AMP-activated protein kinase subunit gamma-3;5'-AMP-activated protein kinase, gamma-1 subunit;5'-AMP-activated protein kinase, gamma-3 subunit;AAKG;AAKG2;AMPK gamma1;AMPK gamma-1 chain;AMPK gamma3;AMPK gamma-3 chain;AMPK subunit gamma-2;AMPK subunit gamma-3;AMPKG;AMPKG3;CMH6;epididymis secretory sperm binding protein;H91620p;protein kinase, AMP-activated, gamma 1 non-catalytic subunit;protein kinase, AMP-activated, gamma 2 non-catalytic subunit;protein kinase, AMP-activated, gamma 3 non-catalytic subunit;SMGMQTL;WPWS. |
NCBI Gene Id | 51422|53632|5571 |
Protein Name | 5'-AMP-activated protein kinase subunit gamma-1|5'-AMP-activated protein kinase subunit gamma-2|5'-AMP-activated protein kinase subunit gamma-3 |
Description of Target | AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive. |
Uniprot ID | P54619|Q9UGI9|Q9UGJ0 |
Protein Accession # | NP_001193638.1 |
Molecular Weight | 37 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "PRKAG1 Antibody (OAAF02319)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "PRKAG1 Antibody (OAAF02319)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "PRKAG1 Antibody (OAAF02319)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "PRKAG1 Antibody (OAAF02319)"?
This target may also be called "5'-AMP-activated protein kinase subunit gamma-1;5'-AMP-activated protein kinase subunit gamma-2;5'-AMP-activated protein kinase subunit gamma-3;5'-AMP-activated protein kinase, gamma-1 subunit;5'-AMP-activated protein kinase, gamma-3 subunit;AAKG;AAKG2;AMPK gamma1;AMPK gamma-1 chain;AMPK gamma3;AMPK gamma-3 chain;AMPK subunit gamma-2;AMPK subunit gamma-3;AMPKG;AMPKG3;CMH6;epididymis secretory sperm binding protein;H91620p;protein kinase, AMP-activated, gamma 1 non-catalytic subunit;protein kinase, AMP-activated, gamma 2 non-catalytic subunit;protein kinase, AMP-activated, gamma 3 non-catalytic subunit;SMGMQTL;WPWS." in publications.
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What is the shipping cost for "PRKAG1 Antibody (OAAF02319)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "PRKAG1 Antibody (OAAF02319)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "PRKAG1 Antibody (OAAF02319)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "37 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "PRKAG1 Antibody (OAAF02319)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "PRKAG1|PRKAG2|PRKAG3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "PRKAG1|PRKAG2|PRKAG3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "PRKAG1|PRKAG2|PRKAG3"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "PRKAG1|PRKAG2|PRKAG3"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "PRKAG1|PRKAG2|PRKAG3"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "PRKAG1|PRKAG2|PRKAG3"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.