Catalog No: OKEH01366
Size:96T
Price: $525.00
SKU
OKEH01366
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PRKACA ELISA Kit (Human) (OKEH01366) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PRKACA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PRKACA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PRKACA captured in well.
ELISA Kit Range0.78-50ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.1% (n = 20)
Mean Inter-assay CV%: < 7.8% (n = 20)
ELISA Kit Component
ComponentAmount
PRKACA Microplate96 Wells (12 x 8 Well strips)
PRKACA Lyophilized Standard2
100X Biotinylated PRKACA Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.34 ng/mL
SpecificityNatural and recombinant Human cAMP-dependent protein kinase catalytic subunit alpha
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPRKACA
Gene Full Nameprotein kinase cAMP-activated catalytic subunit alpha
Alias SymbolsCAFD1;cAMP-dependent protein kinase catalytic subunit alpha;PKA C-alpha;PKACA;PPNAD4;protein kinase A catalytic subunit;protein kinase, cAMP-dependent, alpha catalytic subunit;protein kinase, cAMP-dependent, catalytic, alpha.
NCBI Gene Id5566
Protein NameP17612
Description of TargetcAMP-dependent protein kinase catalytic subunit alpha
Uniprot IDhttps://www.uniprot.org/uniprot/P17612
Protein Accession #NP_001291278.1
Nucleotide Accession #NM_001304349.1
Protein Size (# AA)351
Molecular Weight41 kDa
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