PRKAB1 Antibody (Phospho-Ser182) (OASG00376)

Catalog No: OASG00376

Size:100 uL

Western Blot using COLO205

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: WB, IHC
• Reconstitution and Storage: Store at -20C. Avoid repeated freeze/thaw cycles.
• Immunogen: Synthesized peptide derived from human AMPKBeta1 around the phosphorylation site of S182. Location: 120-200aa.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Concentration: 1 mg/ml
• Target Post-Translational Modification: Phospho-Ser182
• Specificity: Phospho-AMPKBeta1 (S182) Polyclonal Antibody detects endogenous levels of AMPKBeta1 protein only when phosphorylated at S182.
• Application Info: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000;

Target Info

• Gene Symbol: PRKAB1
• Gene Full Name: protein kinase, AMP-activated, beta 1 non-catalytic subunit
• Alias Symbols: AMPK, HAMPKb
• NCBI Gene Id: 5564; 19079; 83803
• Description of Target: The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex.
• Uniprot ID: Q9Y478, Q9R078, P80386
• Molecular Weight: 38 kDa