PRKAB1 Antibody (OAAF00717)

Catalog No: OAAF00717 (Formerly GWB-ASC123)

Size:100 ug

Western blot analysis of extracts from HepG2/Jurkat/HuvEc/MCF-7 cells, using AMPK beta1 Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using AMPK beta1 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Monkey|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human AMPK beta1.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: AMPK β1 (Ab-181) antibody detects endogenous levels of total AMPK β1 protein
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:3000; IHC: 1:50~1:100; ELISA: 1:5000
• Reference: Jun Li, Carcinogenesis, May 2003; 24: 827 - 834.
  Carola U. Niesler, Exp Physiol, Jan 2007; 92: 207 - 217.
  Angela Woods, J. Biol. Chem., Aug 2003; 278: 28434 - 28442.

Target Info

• Gene Symbol: PRKAB1
• Gene Full Name: protein kinase AMP-activated non-catalytic subunit beta 1
• Alias Symbols: 5'-AMP-activated protein kinase beta-1 subunit;5'-AMP-activated protein kinase subunit beta-1;AMP-activated protein kinase beta subunit;AMPK;AMPK beta 1;AMPK beta -1 chain;AMPK subunit beta-1;AMPKb;HAMPKb;protein kinase, AMP-activated, beta 1 non-catalytic subunit;protein kinase, AMP-activated, noncatalytic, beta-1.
• NCBI Gene Id: 5564
• Protein Name: 5'-AMP-activated protein kinase subunit beta-1
• Description of Target: Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).
• Uniprot ID: Q9Y478
• Protein Accession #: NP_006244.2
• Molecular Weight: 30 kDa