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Datasheets/Manuals | Printable datasheet for PRKAB1 Antibody (OAAF00717) |
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Predicted Species Reactivity | Human|Monkey|Mouse|Rat |
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Product Format | Liquid |
Clonality | Polyclonal |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot |
Reconstitution and Storage | -20°C |
Immunogen | The antiserum was produced against synthesized peptide derived from human AMPK beta1. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Specificity | AMPK β1 (Ab-181) antibody detects endogenous levels of total AMPK β1 protein |
Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Dilution | The optimal working dilution should be determined by the end user. |
Application Info | WB: 1:500~1:3000; IHC: 1:50~1:100; ELISA: 1:5000 |
Reference | Jun Li, Carcinogenesis, May 2003; 24: 827 - 834. Carola U. Niesler, Exp Physiol, Jan 2007; 92: 207 - 217. Angela Woods, J. Biol. Chem., Aug 2003; 278: 28434 - 28442. |
Gene Symbol | PRKAB1 |
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Gene Full Name | protein kinase AMP-activated non-catalytic subunit beta 1 |
Alias Symbols | 5'-AMP-activated protein kinase beta-1 subunit;5'-AMP-activated protein kinase subunit beta-1;AMP-activated protein kinase beta subunit;AMPK;AMPK beta 1;AMPK beta -1 chain;AMPK subunit beta-1;AMPKb;HAMPKb;protein kinase, AMP-activated, beta 1 non-catalytic subunit;protein kinase, AMP-activated, noncatalytic, beta-1. |
NCBI Gene Id | 5564 |
Protein Name | 5'-AMP-activated protein kinase subunit beta-1 |
Description of Target | Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3). |
Uniprot ID | Q9Y478 |
Protein Accession # | NP_006244.2 |
Molecular Weight | 30 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "PRKAB1 Antibody (OAAF00717)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Monkey|Mouse|Rat".
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How long will it take to receive "PRKAB1 Antibody (OAAF00717)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "PRKAB1 Antibody (OAAF00717)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "PRKAB1 Antibody (OAAF00717)"?
This target may also be called "5'-AMP-activated protein kinase beta-1 subunit;5'-AMP-activated protein kinase subunit beta-1;AMP-activated protein kinase beta subunit;AMPK;AMPK beta 1;AMPK beta -1 chain;AMPK subunit beta-1;AMPKb;HAMPKb;protein kinase, AMP-activated, beta 1 non-catalytic subunit;protein kinase, AMP-activated, noncatalytic, beta-1." in publications.
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What is the shipping cost for "PRKAB1 Antibody (OAAF00717)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "PRKAB1 Antibody (OAAF00717)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "PRKAB1 Antibody (OAAF00717)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "30 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "PRKAB1 Antibody (OAAF00717)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "PRKAB1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "PRKAB1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "PRKAB1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "PRKAB1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "PRKAB1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "PRKAB1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.