PRKAB1 Antibody (OAAB20868)

Catalog No: OAAB20868

Size:100ul

Western blot detection of AMPK beta 1 in 3T3, Hela, PC-12, COS7 and MDA-MB-468 cell lysates using AMPK beta 1 mouse mAb (OAAB20868) (1:1,000 diluted). Predicted band size: 38KDa. Observed band size: 38KDa. Exposure time: 5min.

Immunohistochemical analysis of paraffin-embedded Breast cancer using AMPK beta 1 mouse mAb (OAAB20868) (1/200 dilution). Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6. 0).

Immunocytochemistry staining of HeLa cells fixed with 1% Paraformaldehyde and using AMPK beta 1 mouse mAb (OAAB20868) (dilution 1:100).

Immunoprecipitation analysis of Hela cell lysates using AMPK beta 1 mouse mAb (OAAB20868).

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Monoclonal
• Host: Rabbit
• Application: WB, ICC, IP, IHC
• Additional Information: Function: Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminal that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Predicted Homology Based on Immunogen Sequence: Human, Mouse
• Preservative: 0.02% Sodium Azide and 50% glycerol
• Formulation: Purified mouse monoclonal antibody in PBS (pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
• Application Info: WB: 1:1000
  ICC: 1:100
  IP: 1:500
  IHC: 1:100
• Reference: Carling D.,et al.Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases.
  Stapleton D.,et al.FEBS Lett. 409:452-456(1997).
  Yamagata K.,et al.Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
  Wang X.,et al.Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases.
  Scherer S.E.,et al.Nature 440:346-351(2006).
  

Target Info

• Gene Symbol: PRKAB1
• Gene Full Name: protein kinase AMP-activated non-catalytic subunit beta 1
• Alias Symbols: AMPK, HAMPKb
• NCBI Gene Id: 5564
• Description of Target: The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex.
• Uniprot ID: Q9Y478
• Protein Accession #: NP_006244.2
• Nucleotide Accession #: NM_006253.4
• Molecular Weight: 30 kDa