Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKEH02064
Size:96T
Price: $525.00
SKU
OKEH02064
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.

PRKAA2 ELISA Kit (Human) : 96 Wells (OKEH02064)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PRKAA2 ELISA Kit (Human) (OKEH02064) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PRKAA2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PRKAA2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PRKAA2 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 100%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.8% (n = 20)
Mean Inter-assay CV%: < 8.1% (n = 20)
ELISA Kit Component
ComponentAmount
PRKAA2 Microplate96 Wells (12 x 8 Well strips)
PRKAA2 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated CYP1A1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.078 ng/mL
SpecificityNatural and recombinant Human 5'-AMP-activated protein kinase catalytic subunit alpha-2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPRKAA2
Gene Full Nameprotein kinase AMP-activated catalytic subunit alpha 2
Alias Symbols5'-AMP-activated protein kinase catalytic subunit alpha-2;5'-AMP-activated protein kinase, catalytic alpha-2 chain;ACACA kinase;acetyl-CoA carboxylase kinase;AMP-activated protein kinase alpha-2 subunit variant 2;AMP-activated protein kinase alpha-2 subunit variant 3;AMPK;AMPK alpha 2;AMPK subunit alpha-2;AMPK2;AMPKa2;AMPK-alpha-2 chain;HMGCR kinase;hydroxymethylglutaryl-CoA reductase kinase;PRKAA;protein kinase, AMP-activated, alpha 2 catalytic subunit.
NCBI Gene Id5563
Protein NameP54646
Description of Target5'-AMP-activated protein kinase catalytic subunit alpha-2
Uniprot IDhttps://www.uniprot.org/uniprot/P54646
Protein Accession #NP_006243.2
Nucleotide Accession #NM_006252.3
Protein Size (# AA)552
Molecular Weight62 kDa
Write Your Own Review
You're reviewing:PRKAA2 ELISA Kit (Human) : 96 Wells (OKEH02064)
Your Rating
We found other products you might like!