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Catalog No: OKCD00690 (Formerly GWB-KBBDP2)
Size:96WELLS
Price: $675.00
SKU
OKCD00690
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Datasheets/ManualsPrintable datasheet for PRH1 MORE ELISA Kit (Human) (OKCD00690)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acidic Salivary Proline Rich Phosphoprotein 2 (PRH2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Acidic Salivary Proline Rich Phosphoprotein 2 (PRH2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Acidic Salivary Proline Rich Phosphoprotein 2 (PRH2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Acidic Salivary Proline Rich Phosphoprotein 2 (PRH2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Acidic Salivary Proline Rich Phosphoprotein 2 (PRH2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Acidic Salivary Proline Rich Phosphoprotein 2 (PRH2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-PRH1 more Microplate96 Wells (12 x 8 Well Strips)
PRH1 more Lyophilized Standard2 x 100 ng
100X Biotinylated PRH1 more Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.55 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Acidic Salivary Proline Rich Phosphoprotein 2 (PRH2). No significant cross-reactivity or interference between Acidic Salivary Proline Rich Phosphoprotein 2 (PRH2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolPRH1|PRH2
Gene Full Nameproline rich protein HaeIII subfamily 1|proline rich protein HaeIII subfamily 2
Alias Symbolsacidic salivary proline-rich protein, HaeIII type, 2;Db-s;PA;parotid acidic protein;parotid double-band protein;parotid isoelectric focusing variant protein;parotid proline-rich protein 1/2;PIF-S;Pr;Pr1/Pr2;PRH2;protein C;PRP-1/PRP-2;salivary acidic proline-rich phosphoprotein 1/2.
NCBI Gene Id5554|5555
Protein NameSalivary acidic proline-rich phosphoprotein 1/2
Description of TargetPRP's act as highly potent inhibitors of crystal growth of calcium phosphates. They provide a protective and reparative environment for dental enamel which is important for the integrity of the teeth.
Uniprot IDP02810
Protein Accession #NP_001103683
Nucleotide Accession #NM_001110213.1
Protein Size (# AA)166
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