Catalog No: OKCD00690 (Formerly GWB-KBBDP2)
Size:96WELLS
Price: $675.00
SKU
OKCD00690
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.
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- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
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Datasheets/Manuals | Printable datasheet for PRH1 MORE ELISA Kit (Human) (OKCD00690) |
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Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acidic Salivary Proline Rich Phosphoprotein 2 (PRH2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Acidic Salivary Proline Rich Phosphoprotein 2 (PRH2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Acidic Salivary Proline Rich Phosphoprotein 2 (PRH2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Acidic Salivary Proline Rich Phosphoprotein 2 (PRH2) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Acidic Salivary Proline Rich Phosphoprotein 2 (PRH2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Acidic Salivary Proline Rich Phosphoprotein 2 (PRH2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates, cell lysates and other biological fluids | ||||||||||||||||||||||
Sensitivity | < 0.55 ng/mL | ||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of Acidic Salivary Proline Rich Phosphoprotein 2 (PRH2). No significant cross-reactivity or interference between Acidic Salivary Proline Rich Phosphoprotein 2 (PRH2) and analogues was observed. | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich Immunoassay |
Gene Symbol | PRH1|PRH2 |
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Gene Full Name | proline rich protein HaeIII subfamily 1|proline rich protein HaeIII subfamily 2 |
Alias Symbols | acidic salivary proline-rich protein, HaeIII type, 2;Db-s;PA;parotid acidic protein;parotid double-band protein;parotid isoelectric focusing variant protein;parotid proline-rich protein 1/2;PIF-S;Pr;Pr1/Pr2;PRH2;protein C;PRP-1/PRP-2;salivary acidic proline-rich phosphoprotein 1/2. |
NCBI Gene Id | 5554|5555 |
Protein Name | Salivary acidic proline-rich phosphoprotein 1/2 |
Description of Target | PRP's act as highly potent inhibitors of crystal growth of calcium phosphates. They provide a protective and reparative environment for dental enamel which is important for the integrity of the teeth. |
Uniprot ID | P02810 |
Protein Accession # | NP_001103683 |
Nucleotide Accession # | NM_001110213.1 |
Protein Size (# AA) | 166 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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