PRDM16 Antibody (OAPB01020)

Catalog No: OAPB01020

Size:100UG

Western blot analysis of PRDM16 in rat brain tissue lysate with PRDM16 antibody at (A) 1 and (B) 2 μg/mL.

Immunohistochemistry of PRDM16 in rat brain tissue with PRDM16 antibody at 2.5 μg/mL.

Immunofluorescence of PRDM16 in Human Brain cells with PRDM16 antibody at 20 ug/mL.

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Liquid. PRDM16 Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: 2°C to 8°C
• Immunogen: PRDM16 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human PRDM16. The immunogen is located within amino acids 1120 - 1170 of PRDM16.
• Purification: Affinity Purified
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-PRDM16 antibody is Catalog # OEPB03135
• Application Info: PRDM16 antibody can be used for detection of PRDM16 by Western blot at 1 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 ug/mL.
• Reference: Mochizuki N, Shimizu S, Nagasawa T, et al. A novel gene, MEL1, mapped to 1p36.3 is highly homologous to the MDS1/EVI1 gene and is transcriptionally activated in t(1;3)(p36;q21)-positive leukemia cells. Blood2000; 96:3209-14.;Morishita K. Leukemogenesis of the EVI1/MEL1 gene family. Int. J. Hematol.2007; 85:279-86.;Warner DR, Horn KH, Mudd L, et al. PRDM16/MEL1: a novel Smad binding protein expressed in murine embryonic orofacial tissue. Biochim. Biophys. Acta2007; 1773:814-20.;Seale P, Bjork B, Yang W, et al. PRDM16 controls a brown fat/skeletal muscle switch. Nature2008; 454:961-7.

Target Info

• Gene Symbol: PRDM16
• Gene Full Name: PR/SET domain 16
• Alias Symbols: CMD1LL;histone-lysine N-methyltransferase PRDM16;KMT8F;LVNC8;MDS1/EVI1-like gene 1;MEL1;PFM13;PR domain 16;PR domain containing 16;PR domain zinc finger protein 16;PR domain-containing protein 16;transcription factor MEL1.
• NCBI Gene Id: 63976
• Protein Name: PR domain containing 16 [Homo sapiens]|PR domain zinc finger protein 16
• Description of Target: Binds DNA and functions as a transcriptional regulator. Functions in the differentiation of brown adipose tissue (BAT) which is specialized in dissipating chemical energy in the form of heat in response to cold or excess feeding while white adipose tissue (WAT) is specialized in the storage of excess energy and the control of systemic metabolism. Together with CEBPB, regulates the differentiation of myoblastic precursors into brown adipose cells. Functions also as a repressor of TGF-beta signaling. Isoform 4 may regulate granulocytes differentiation.
• Uniprot ID: Q9HAZ2
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/CAH71530
• Molecular Weight: Predicted: 109, 120, 140 kDa Observed: 140 kDa