PRCP ELISA Kit (Rat) (OKDD00482)

Catalog No: OKDD00482 (Formerly GWB-KBBUC1)

Size:96 Wells

Rat PRCP ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked immunosorbent assay
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~4 Hours
• ELISA Kit Principle: Aviva Systems Biology PRCP ELISA Kit (Rat) (OKDD00482) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for PRCP has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PRCP is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample PRCP captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility:

  Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PRCP were tested 20 times on one plate, respectively.

  Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PRCP were tested on 3 different plates, 8 replicates in each plate.

  Intra-Assay: CV<10%

  Inter-Assay: CV<12%

• ELISA Kit Component:
  • Anti-PRCP Microplate: 96 Wells (12 x 8 Well Strips)
  • PRCP Lyophlized Standard: 2 x 10 ng
  • Standard Diluent: 1 x 20 mL
  • 100X PRCP Biotinylated Detector Antibody: 1 x 120 ÂuL
  • 100X Avidin-HRP Detection Conjugate: 1 x 120 ÂuL
  • Detector Antibody Diluent: 1 x 12 mL
  • Detection Conjugate Diluent: 1 x 12 mL
  • 10X Sample Diluent: 1 x 30 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates
• Sensitivity: < 0.042 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of PRCP. No significant cross-reactivity or interference between PRCP and analogues was observed.
• Assay Info: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Prcp
• Gene Full Name: prolylcarboxypeptidase
• Alias Symbols: lysosomal Pro-X carboxypeptidase;prolylcarboxypeptidase (angiotensinase C).
• NCBI Gene Id: 293118
• Protein Name: Prolylcarboxypeptidase
• Uniprot ID: D4AA31
• Protein Accession #: NP_001099751.2
• Nucleotide Accession #: NM_001106281.2
• Protein Size (# AA): 493
• Molecular Weight: 56 kDa