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Datasheets/Manuals | Printable datasheet for PRAM1 Antibody (OAEB02272) |
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Predicted Species Reactivity | Human|Porcine |
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Product Format | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Clonality | Polyclonal |
Host | Goat |
Application | WB |
Additional Information | Reactivity Info: Tested: Human; Expected: Human |
Reconstitution and Storage | Aliquot and store at -20C. Minimize freezing and thawing. |
Immunogen | PRAM1 |
Purification | Ammonium Sulfate Precipitated; Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Human |
Peptide Sequence | DFCDPLENQPLPLGR |
Concentration | 0.5 mg/ml |
Purity | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Application Info | Peptide ELISA: antibody detection limit dilution 1:8000. Western Blot: Approx 90-100kDa band seen in Jurkat cell lysates. Recommended for use at 1-3ug/ml. Overnight incubation is recommended with this antibody. Please note that although the predicted MW of PRAM-1 is 79kDa, Moog-Lutz et al., (J. Biol. Chem, Jun 2001; 276: 22375 - 22381) detected aPRAM-1 migrating with an apparent molecular mass of 97 kDa and this is what we observed as well. |
Reference | * Moog-Lutz C, Peterson EJ, Lutz PG, Eliason S, Cave-Riant F, Singer A, Di Gioia Y, Dmowski S, Kamens J, Cayre YE, Koretzky G.; PRAM-1 is a novel adaptor protein regulated by retinoic acid (RA) and promyelocytic leukemia (PML)-RA receptor alpha in acute promyelocytic leukemia cells.; J Biol Chem 2001 Jun 22;276(25):22375-81; Pubmed 11301322 |
Gene Symbol | PRAM1 |
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Gene Full Name | PML-RARA regulated adaptor molecule 1 |
Alias Symbols | PML-RAR;PML-RARA target gene encoding an Adaptor Molecule-1;PML-RARA-regulated adapter molecule 1;PRAM;PRAM-1. |
NCBI Gene Id | 84106 |
Protein Name | PML-RARA-regulated adapter molecule 1 |
Uniprot ID | Q96QH2 |
Protein Accession # | NP_115528.3 |
Molecular Weight | 74 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "PRAM1 Antibody (OAEB02272)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Porcine".
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How long will it take to receive "PRAM1 Antibody (OAEB02272)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "PRAM1 Antibody (OAEB02272)" provided in?
This item is provided in "Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "PRAM1 Antibody (OAEB02272)"?
This target may also be called "PML-RAR;PML-RARA target gene encoding an Adaptor Molecule-1;PML-RARA-regulated adapter molecule 1;PRAM;PRAM-1." in publications.
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What is the shipping cost for "PRAM1 Antibody (OAEB02272)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "PRAM1 Antibody (OAEB02272)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "PRAM1 Antibody (OAEB02272)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "74".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "PRAM1 Antibody (OAEB02272)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "PRAM1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "PRAM1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "PRAM1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "PRAM1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "PRAM1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "PRAM1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.