Catalog No: OKEH01952
Size:96T
Price: $625.00
SKU
OKEH01952
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityPorcine, Sus scrofa
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PR39 ELISA Kit (Pig) (OKEH01952) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-pig PR39 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated PR39 and incubated. The PR39 found in the sample or standards competes with the biotinylated PR39 for limited binding sites on the immobilized anti-pig PR39 antibody. Excess unbound biotinylated PR39 and sample or standard PR39 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated PR39 captured in the well and inversely proportional to the amount of PR39 which was contained in the sample or standard.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.8% (n = 20)
Mean Inter-assay CV%: < 8.7% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-PR39 Microplate96 Wells (12 x 8 Well strips)
PR39 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X PR39-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity24 pg/mL
SpecificityNatural and recombinant Pig Antibacterial protein PR-39
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPR39
Gene Full Namepeptide antibiotic PR39
Alias Symbolsantibacterial protein PR-39;cathelicidin;PR-39.
NCBI Gene Id407610
Protein NameP80054
Description of TargetAntibacterial protein PR-39
Uniprot IDhttps://www.uniprot.org/uniprot/P80054
Protein Accession #NP_999615.1
Nucleotide Accession #NM_214450.1
Protein Size (# AA)172
Molecular Weight19 kDa
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