Catalog No: OKEH01979
Size:96T
Price: $600.00
SKU
OKEH01979
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH01979
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PPP1CA ELISA Kit (Human) (OKEH01979) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PPP1CA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PPP1CA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PPP1CA captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 94%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.6% (n = 20)
Mean Inter-assay CV%: < 8.0% (n = 20)
ELISA Kit Component
ComponentAmount
PPP1CA Microplate96 Wells (12 x 8 Well strips)
PPP1CA Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated PPP1CA Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.055 ng/mL
SpecificityNatural and recombinant Human Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPPP1CA
Gene Full Nameprotein phosphatase 1 catalytic subunit alpha
Alias SymbolsPP1A;PP-1A;PP1alpha;PPP1A;protein phosphatase 1, catalytic subunit, alpha isozyme;serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit;serine/threonine-protein phosphatase PP1-alpha catalytic subunit;testicular tissue protein Li 155.
NCBI Gene Id5499
Protein NameP62136
Description of TargetSerine/threonine-protein phosphatase PP1-alpha catalytic subunit
Uniprot IDhttps://www.uniprot.org/uniprot/P62136
Protein Accession #NP_001008709.1
Nucleotide Accession #NM_001008709.1
Protein Size (# AA)330
Molecular Weight37 kDa
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