PPME1 ELISA Kit (Rat) (OKEH03629)

Catalog No: OKEH03629

Size:96T

Rat PPME1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology PPME1 ELISA Kit (Rat) (OKEH03629) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human Ppme1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Ppme1 and incubated. The Ppme1 found in the sample or standards competes with the biotinylated Ppme1 for limited binding sites on the immobilized anti-human Ppme1 antibody. Excess unbound biotinylated Ppme1 and sample or standard Ppme1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Ppme1 captured in the well and inversely proportional to the amount of Ppme1 which was contained in the sample or standard.
• ELISA Kit Range: 0.625-40ng/mL
• ELISA Kit Component:
  • Anti-Ppme1 Microplate: 96 Wells (12 x 8 Well strips)
  • Ppme1 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Ppme1-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.325 ng/mL
• Specificity: Natural and recombinant Rat Protein phosphatase methylesterase 1
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: Ppme1
• Gene Full Name: protein phosphatase methylesterase 1
• Alias Symbols: PME-1;protein phosphatase methylesterase 1;RGD1309683.
• NCBI Gene Id: 361613
• Protein Name: Q4FZT2
• Description of Target: Protein phosphatase methylesterase 1
• Uniprot ID: https://www.uniprot.org/uniprot/Q4FZT2
• Protein Accession #: NP_001178767.1
• Nucleotide Accession #: NM_001191838.1
• Protein Size (# AA): 386
• Molecular Weight: 42 kDa