Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKEH00825
Size:96T
Price: $600.00
SKU
OKEH00825
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.

PPARGC1A ELISA Kit (Rat) : 96 Wells (OKEH00825)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PPARGC1A ELISA Kit (Rat) (OKEH00825) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PPARGC1A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PPARGC1A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PPARGC1A captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.9% (n = 20)
Mean Inter-assay CV%: < 8.4% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-PPARGC1A Microplate96 Wells (12 x 8 Well strips)
PPARGC1A Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated PPARGC1A Detector Antibody1 x 120 ul
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.078 ng/mL
SpecificityNatural and recombinant Rat Peroxisome proliferator-activated receptor gamma coactivator 1-alpha
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPpargc1a
Gene Full NamePPARG coactivator 1 alpha
Alias SymbolsLRPGC1;peroxisome proliferative activated receptor gamma coactivator 1;peroxisome proliferative activated receptor, gamma, coactivator 1 alpha;peroxisome proliferator-activated receptor gamma coactivator 1-alpha;PGC-1alpha;PGC-1-alpha;PGC-1v;PGCvf;PGCvf1;PGCvf-1;PPAR gamma coactivator 1alpha variant form-1;PPAR-gamma coactivator 1-alpha;Ppargc1;PPARGC-1-alpha;Ppargc1a-vf1.
NCBI Gene Id83516
Protein NameQ9QYK2
Description of TargetPeroxisome proliferator-activated receptor gamma coactivator 1-alpha
Uniprot IDhttps://www.uniprot.org/uniprot/Q9QYK2
Protein Accession #NP_112637.1
Nucleotide Accession #NM_031347.1
Protein Size (# AA)796
Molecular Weight90 kDa
Write Your Own Review
You're reviewing:PPARGC1A ELISA Kit (Rat) : 96 Wells (OKEH00825)
Your Rating
We found other products you might like!