PPARGC1A Antibody (OAEB02003)

Catalog No: OAEB02003 (Formerly GWB-4AA3E2)

Size:100UG

(0.3ug/ml) staining of Rat Stomach lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

OAEB02003 (0.1ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stai

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stai

Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fo

Product Info

• Tested Species Reactivity: Human, Rat
• Predicted Species Reactivity: Bovine|Dog|Human|Mouse|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB, P-ELISA, IF, FC
• Reconstitution and Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Immunogen: PPARGC1A
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Peptide Sequence: C-DGLFDDSEDESDK
• Concentration: 0.5 mg/ml
• Application Info: P-ELISA: Antibody detection limit dilution 1:128000
  WB: Approx 100kDa band observed in Rat Duodenum lysates (calculated MW of 91.0kDa according to NP_037393.1). This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1.0-3.0 ug/ml. Primary incubation 1 hour at room temperature.
  IF: Strong expression of the protein seen in the nuclei and cytoplasm of HeLa and U2OS cells. Recommended concentration: 10 ug/ml.
  FC: Flow cytometric analysis of HeLa cells. Recommended concentration: 10 ug/ml.
• Reference: * Handschin C, Kobayashi YM, Chin S, Seale P, Campbell KP, Spiegelman BM.; PGC-1alpha regulates the neuromuscular junction program and ameliorates Duchenne muscular dystrophy.; Genes Dev. 2007 Apr 1;21(7):770-83.; Pubmed 17403779
• Publications:

  Spelman, K. et al. Role for PPARgamma in IL-2 inhibition in T cells by Echinacea-derived undeca-2E-ene-8,10-diynoic acid isobutylamide. Int. Immunopharmacol. 9, 1260-4 (2009). 19712756

Target Info

• Gene Symbol: Ppargc1a|PPARGC1A
• Gene Full Name: peroxisome proliferative activated receptor, gamma, coactivator 1 alpha|PPARG coactivator 1 alpha
• Alias Symbols: A830037N07Rik;Gm11133;LEM6;ligand effect modulator-6;L-PGC-1alpha;LRPGC1;peroxisome proliferative activated receptor gamma coactivator 1;peroxisome proliferative activated receptor, gamma, coactivator 1 alpha;peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4-3ext;peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4-8a;peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B5-NT;peroxisome proliferator-activated receptor gamma coactivator 1-alpha;Pgc;PGC1;PGC-1;PGC-1(alpha);PGC1A;PGC-1alpha;PGC-1-alpha;PGC-1v;Pgco1;PGCvf;PGCvf1;PGCvf-1;PPAR gamma coactivator 1alpha variant form-1;PPAR gamma coactivator variant form;PPARgamma coactivator 1alpha;PPAR-gamma coactivator 1-alpha;Ppargc1;PPARGC-1-alpha;Ppargc1a-vf1.
• NCBI Gene Id: 10891|19017|83516
• Protein Name: Peroxisome proliferator-activated receptor gamma coactivator 1-alpha
• Uniprot ID: Q4W5M7
• Protein Accession #: NP_037393.1
• Molecular Weight: 91.0; 91.7; 89.6; 77.1; 91.5