Catalog No: OKEH01445
Size:96T
Price: $525.00
SKU
OKEH01445
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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PPARD ELISA Kit (Human) : 96 Wells (OKEH01445)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PPARD ELISA Kit (Human) (OKEH01445) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PPARD has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PPARD is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PPARD captured in well.
ELISA Kit Range31.2-2000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.6% (n = 20)
Mean Inter-assay CV%: < 9.6% (n = 20)
ELISA Kit Component
ComponentAmount
PPARD Microplate96 Wells (12 x 8 Well strips)
PPARD Lyophilized Standard2
100X Biotinylated PPARD Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity10 pg/mL
SpecificityNatural and recombinant Human Peroxisome proliferator-activated receptor delta
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPPARD
Gene Full Nameperoxisome proliferator activated receptor delta
Alias SymbolsFAAR;NR1C2;NUC1;NUCI;NUCII;nuclear hormone receptor 1;nuclear receptor subfamily 1 group C member 2;peroxisome proliferator-activated receptor beta;peroxisome proliferator-activated receptor delta;PPARB;PPAR-beta;PPARD/MYO1D fusion;PPAR-delta.
NCBI Gene Id5467
Protein NameQ03181
Description of TargetPeroxisome proliferator-activated receptor delta
Uniprot IDhttps://www.uniprot.org/uniprot/Q03181
Protein Accession #NP_001165289.1
Nucleotide Accession #NM_001171818.1
Protein Size (# AA)441
Molecular Weight50 kDa
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