Catalog No: P100956_T100
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P100956_T100
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Datasheets/ManualsPrintable datasheet for anti-POU5F1 (P100956_T100) antibody
Product Info
Tested Species ReactivityHuman
Predicted Species ReactivityHuman, Dog, Goat, Pig, Rabbit
Product FormatLiquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
ClonalityPolyclonal
HostRabbit
ApplicationWB
Reconstitution and StorageFor short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
ImmunogenThe immunogen is a synthetic peptide directed towards the N terminal region of human POU5F1
PurificationProtein A purified
Predicted Homology Based on Immunogen SequenceDog: 91%; Goat: 100%; Human: 100%; Pig: 84%; Rabbit: 92%
Peptide SequenceSynthetic peptide located within the following region: PCTVTPGAVKLEKEKLEQNPEESQDIKALQKELEQFAKLLKQKRITLGYT
Concentration1.0 mg/ml
Blocking PeptideFor anti-POU5F1 (P100956_T100) antibody is Catalog # AAP31313 (Previous Catalog # AAPP02063)
ReferenceSuo,G., et al., (2005) Biochem. Biophys. Res. Commun. 337 (4), 1047-1051
Publications

Kaeffer, B. et al. Non-invasive exploration of neonatal gastric epithelium by using exfoliated epithelial cells. PLoS One 6, e25562 (2011). 22028779

Li, Y. et al. BMP4-directed trophoblast differentiation of human embryonic stem cells is mediated through a dNp63+ cytotrophoblast stem cell state. Development 140, 3965-76 (2013). 24004950

Oct-4B isoform is differentially expressed in breast cancer cells: hypermethylation of regulatory elements of Oct-4A suggests an alternative promoter and transcriptional start site for Oct-4B transcription. Biosci Rep. 31, 109-15 (2011). 20433421

Description
Gene SymbolPOU5F1
Gene Full NamePOU class 5 homeobox 1
Alias SymbolsOCT3, OCT4, OTF3, OTF4, OTF-3, Oct-3, Oct-4
NCBI Gene Id5460
Protein NamePOU domain, class 5, transcription factor 1
Description of TargetPOU5F1 is a POU transcription factor expressed by early embryo cells and germ cells. It determines paracrine growth factor signaling from stem cells to the trophectoderm
Uniprot IDQ01860
Protein Accession #NP_002692
Nucleotide Accession #NM_002701
Protein Size (# AA)360
Molecular Weight39kDa
Protein InteractionsETS2; CETN2; XPC; RAD23B; UBC; ITCH; FOXD3; WWP2; PSMD10; NCL; PURA; PPIA; POU5F1; YBX1; KRT19; KRT14; KRT10; KRT9; KRT2; KRT1; HNRNPK; HMGB1; HIST1H1T; GAPDH; FKBP7; EHMT2; AGR2; TUBB4A; EIF5B; TUBA1A; NR2F2; TRA2B; SRSF3; RPS25; RPS23; RPS19; RPL31; RPL
  1. What is the species homology for "POU5F1 Antibody - N-terminal region (P100956_T100)"?

    The tested species reactivity for this item is "Human". This antibody is predicted to have homology to "Human, Dog, Goat, Pig, Rabbit".

  2. How long will it take to receive "POU5F1 Antibody - N-terminal region (P100956_T100)"?

    This item is available "Domestic: within 1-2 days delivery | International: 1-2 days".

  3. What buffer format is "POU5F1 Antibody - N-terminal region (P100956_T100)" provided in?

    This item is provided in "Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "POU5F1 Antibody - N-terminal region (P100956_T100)"?

    This target may also be called "OCT3, OCT4, OTF3, OTF4, OTF-3, Oct-3, Oct-4" in publications.

  5. What is the shipping cost for "POU5F1 Antibody - N-terminal region (P100956_T100)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "POU5F1 Antibody - N-terminal region (P100956_T100)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "POU5F1 Antibody - N-terminal region (P100956_T100)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "39kDa".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "POU5F1 Antibody - N-terminal region (P100956_T100)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "POU5F1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "POU5F1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "POU5F1"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "POU5F1"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "POU5F1"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "POU5F1"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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