Catalog No: OKCD02813 (Formerly GWB-JQUNFW)
Size:96WELLS
Price: $625.00
SKU
OKCD02813
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Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)81-88%89-98%80-90%91-101%
EDTA Plasma (n=5)91-99%95-103%84-95%82-103%
Heparin Plasma (n=5)87-101%94-101%84-98%81-95%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Paraoxonase 1 (PON1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Paraoxonase 1 (PON1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Paraoxonase 1 (PON1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Paraoxonase 1 (PON1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range125-8,000ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)86-9892
EDTA Plasma (n=5)84-10194
Heparin Plasma(n=5)95-105101
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Paraoxonase 1 (PON1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Paraoxonase 1 (PON1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-PON1 Microplate96 Wells (12 x 8 Well strips)
PON1 Lyophilized Standard2
100X Biotinylated PON1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture cell supernatant and other biological fluids
Sensitivity0.51 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Paraoxonase 1 (PON1). No significant cross-reactivity or interference between Paraoxonase 1 (PON1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolPon1
Gene Full Nameparaoxonase 1
Alias SymbolsA-esterase 1;aromatic esterase 1;Pon;PON 1;serum aryldialkylphosphatase 1;serum paraoxonase/arylesterase 1.
NCBI Gene Id18979
Protein NameSerum paraoxonase/arylesterase 1
Description of TargetHydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification.
Uniprot IDP52430
Protein Accession #NP_035264.2
Nucleotide Accession #NM_011134.3
Protein Size (# AA)355
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