Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKEH00331
Size:96T
Price: $525.00
SKU
OKEH00331
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.

PON1 ELISA Kit (Human) : 96 Wells (OKEH00331)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PON1 ELISA Kit (Human) (OKEH00331) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PON1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for PON1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PON1 captured in well.
ELISA Kit Range1.56-100ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 85%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 3.6% (n = 20)
Mean Inter-assay CV%: < 7.2% (n = 20)
ELISA Kit Component
ComponentAmount
PON1 Microplate96 Wells (12 x 8 Well strips)
PON1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated PON1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.78 ng/mL
SpecificityNatural and recombinant Human Serum paraoxonase/arylesterase 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPON1
Gene Full Nameparaoxonase 1
Alias SymbolsA-esterase 1;aromatic esterase 1;arylesterase 1;arylesterase B-type;ESA;esterase A;K-45;MVCD5;paraoxonase B-type;PON;PON 1;serum aryldiakylphosphatase;serum aryldialkylphosphatase 1;serum paraoxonase/arylesterase 1.
NCBI Gene Id5444
Protein NameP27169
Description of TargetSerum paraoxonase/arylesterase 1
Uniprot IDhttps://www.uniprot.org/uniprot/P27169
Protein Accession #NP_000437.3
Nucleotide Accession #NM_000446.5
Protein Size (# AA)355
Molecular Weight40 kDa
Write Your Own Review
You're reviewing:PON1 ELISA Kit (Human) : 96 Wells (OKEH00331)
Your Rating
We found other products you might like!