Catalog No: OKEH00837
Size:96T
Price: $525.00
SKU
OKEH00837
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology POMT1 ELISA Kit (Human) (OKEH00837) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for POMT1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for POMT1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample POMT1 captured in well.
ELISA Kit Range0.78-50ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 92%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.1% (n = 20)
Mean Inter-assay CV%: < 8.5% (n = 20)
ELISA Kit Component
ComponentAmount
POMT1 Microplate96 Wells (12 x 8 Well strips)
POMT1 Lyophilized Standard2
100X Biotinylated POMT1 Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.22 ng/mL
SpecificityNatural and recombinant Human Protein O-mannosyl-transferase 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPOMT1
Gene Full Nameprotein O-mannosyltransferase 1
Alias Symbolsdolichyl-phosphate-mannose--protein mannosyltransferase 1;LGMD2K;LGMDR11;MDDGA1;MDDGB1;MDDGC1;protein O-mannosyl-transferase 1;RT;testis tissue sperm-binding protein Li 57p;truncated O-mannosyl-transferase 1 variant SV3DEL.
NCBI Gene Id10585
Protein NameQ9Y6A1
Description of TargetProtein O-mannosyl-transferase 1
Uniprot IDhttps://www.uniprot.org/uniprot/Q9Y6A1
Protein Accession #NP_001070833.1
Nucleotide Accession #NM_001077365.1
Protein Size (# AA)747
Molecular Weight85 kDa
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