POMC ELISA Kit (Rat) (OKCD02777)

Catalog No: OKCD02777

Size:96WELLS

POMC ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 2h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	79-101%	91-99%	80-97%	80-90%
  EDTA Plasma (n=5)	95-104%	85-99%	82-101%	86-99%
  Heparin Plasma (n=5)	84-99%	80-90%	80-90%	80-103%

• ELISA Kit Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Proopiomelanocortin (POMC) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Proopiomelanocortin (POMC) and unlabeled Proopiomelanocortin (POMC) (Standards or samples) with the pre-coated antibody specific to Proopiomelanocortin (POMC). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Proopiomelanocortin (POMC) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Proopiomelanocortin (POMC) in the sample.
• ELISA Kit Range: 123.5-10,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	98-105	102
  EDTA Plasma (n=5)	99-105	102
  Heparin Plasma (n=5)	93-105	99

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Proopiomelanocortin (POMC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Proopiomelanocortin (POMC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Pomc Microplate: 96 Wells (12 x 8 Well strips)
  • Pomc Lyophilized Standard: 2 x 10 ng
  • 100X Pomc Biotin Complex: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 52.5 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Proopiomelanocortin (POMC). No significant cross-reactivity or interference between Proopiomelanocortin (POMC) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Competitive Inhibition ELISA

Target Info

• Gene Symbol: POMC
• Gene Full Name: Proopiomelanocortin
• Alias Symbols: Corticotropin-lipotropin.
• NCBI Gene Id: 24664
• Protein Name: Pro-opiomelanocortin
• Description of Target: Corticotropin: Stimulates the adrenal glands to release cortisol.
• Uniprot ID: P01194
• Protein Accession #: XP_017449522.1
• Nucleotide Accession #: XM_017594033.1
• Molecular Weight: 235 kDa