POLR2B Antibody (OAGA00187)

Catalog No: OAGA00187

Size:100 ul

Sample (30 ug of whole cell lysate)
A: BCL-1
5% SDS PAGE
OAGA00187 diluted at 1:1000

Sample (30 ug of whole cell lysate)
A: H1299
5% SDS PAGE
OAGA00187 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using POLR2B(OAGA00187) antibody at 1:500 dilution.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using POLR2B(OAGA00187) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid 0.1M Tris-Glycine, 20% glycerol (pH 7) with 0.01% Thimerosal
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-P, WB, CHIP
• Additional Information: Positive Controls: H1299, BCL-1
  Cellular Localization: Nucleus
• Reconstitution and Storage: Keep as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term store at -20°C or below. Avoid multiple-freeze thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human RPB2. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.32 mg/ml
• Application Info: WB: 1:500~3000
  ICC/IF: 1:100~1000
  IHC-P: 1:100~1000
  CHIP: Assay dependent

Target Info

• Gene Symbol: POLR2B
• Gene Full Name: DNA-directed RNA polymerase II subunit RPB2
• Alias Symbols: RPB2, POL2RB, hRPB140
• NCBI Gene Id: 5431
• Protein Name: DNA-directed RNA polymerase II subunit RPB2
• Description of Target: This gene encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA.
• Uniprot ID: P30876
• Protein Accession #: NP_000929.1
• Molecular Weight: 134 kDa