Catalog No: OKEH02869
Size:96T
Price: $600.00
SKU
OKEH02869
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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PODXL ELISA Kit (Human) : 96 Wells (OKEH02869)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH02869
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PODXL ELISA Kit (Human) (OKEH02869) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PODXL has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PODXL is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PODXL captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 94%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.5% (n = 20)
Mean Inter-assay CV%: < 7.8% (n = 20)
ELISA Kit Component
ComponentAmount
PODXL Microplate96 Wells (12 x 8 Well strips)
PODXL Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated PODXL Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.1 ng/mL
SpecificityNatural and recombinant Human Podocalyxin
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPODXL
Gene Full Namepodocalyxin like
Alias SymbolsGCTM-2 antigen;gp135;Gp200;PC;PCLP;PCLP-1;PDX;podocalyxin;podocalyxin-like protein 1;PODXL1.
NCBI Gene Id5420
Protein NameO00592
Description of TargetPodocalyxin
Uniprot IDhttps://www.uniprot.org/uniprot/O00592
Protein Accession #NP_001018121.1
Nucleotide Accession #NM_001018111.2
Protein Size (# AA)558
Molecular Weight58 kDa
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