Catalog No: OKCD02811
Size:96WELLS
Price: $700.00
SKU
OKCD02811
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
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Datasheets/Manuals | Printable datasheet for PMP22 ELISA Kit (Mouse) (OKCD02811) |
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Predicted Species Reactivity | Mouse|Mus musculus | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peripheral Myelin Protein 22 (PMP22). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peripheral Myelin Protein 22 (PMP22). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peripheral Myelin Protein 22 (PMP22), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Peripheral Myelin Protein 22 (PMP22) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peripheral Myelin Protein 22 (PMP22) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peripheral Myelin Protein 22 (PMP22) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates, cell lysates and other biological fluids | ||||||||||||||||||||||
Sensitivity | < 0.057 ng/mL | ||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of Peripheral Myelin Protein 22 (PMP22). No significant cross-reactivity or interference between Peripheral Myelin Protein 22 (PMP22) and analogues was observed. | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA | ||||||||||||||||||||||
Protocol Information |
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Gene Symbol | Pmp22 |
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Gene Full Name | peripheral myelin protein 22 |
Alias Symbols | 22kDa;Gas;Gas-3;growth arrest-specific protein 3;HNPP;peripheral myelin protein 22;peripheral myelin protein, 22 kDa;PMP-22;Tr;TRE00;TRE002;trembler. |
NCBI Gene Id | 18858 |
Protein Name | Peripheral myelin protein 22 |
Description of Target | Might be involved in growth regulation, and in myelinization in the peripheral nervous system. |
Uniprot ID | P16646 |
Protein Accession # | NP_001289184.1 |
Nucleotide Accession # | NM_001302255.1 |
Protein Size (# AA) | 161 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
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