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Datasheets/Manuals | Printable datasheet for OAAB15905 |
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Predicted Species Reactivity | Human |
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Product Format | Liquid. PBS with 0.09% (W/V) sodium azide. |
Clonality | Polyclonal |
Isotype | Ig |
Host | Rabbit |
Application | WB, IHC-P, IF, FC |
:: | Function: Key component of PML nuclear bodies that regulate a large number of cellular processes by facilitating post- translational modification of target proteins, promoting protein- protein contacts, or by sequestering proteins. Functions as tumor suppressor. Required for normal, caspase-dependent apoptosis in response to DNA damage, FAS, TNF, or interferons. Plays a role in transcription regulation, DNA damage response, DNA repair and chromatin organization. Plays a role in processes regulated by retinoic acid, regulation of cell division, terminal differentiation of myeloid precursor cells and differentiation of neural progenitor cells. Required for normal immunity to microbial infections. Plays a role in antiviral response. In the cytoplasm, plays a role in TGFB1-dependent processes. Regulates p53/TP53 levels by inhibiting its ubiquitination and proteasomal degradation. Regulates activation of p53/TP53 via phosphorylation at 'Ser-20'. Sequesters MDM2 in the nucleolus after DNA damage, and thereby inhibits ubiquitination and degradation of p53/TP53 Regulates translation of HIF1A by sequestering MTOR, and thereby plays a role in neoangiogenesis and tumor vascularization Regulates RB1 phosphorylation and activity. Required for normal development of the brain cortex during embryogenesis. Can sequester herpes virus and varicella virus proteins inside PML bodies, and thereby inhibit the formation of infectious viral particles. Regulates phosphorylation of ITPR3 and plays a role in the regulation of calcium homeostasis at the endoplasmic reticulum (By similarity). Regulates transcription activity of ELF4 Inhibits specifically the activity of the tetrameric form of PKM Together with SATB1, involved in local chromatin-loop remodeling and gene expression regulation at the MHC-I locus. Regulates PTEN compartmentalization through the inhibition of USP7-mediated deubiquitination. Cellular Location: Nucleus, nucleoplasm. Cytoplasm. Nucleus, PML body. Nucleus, nucleolus. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side (By similarity) Early endosome membrane; Peripheral membrane protein; Cytoplasmic side. Note=Sumoylated forms localize to the PML nuclear bodies The B1 box and the RING finger are also required for this nuclear localization. Isoforms lacking a nuclea. |
Reconstitution and Storage | Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
Immunogen | This PML Sumoylation Site antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 470-499 amino acids from human PML Sumoylation Site. |
Purification | This antibody is purified through a protein A column, followed by peptide affinity purification. |
Predicted Homology Based on Immunogen Sequence | Human |
Concentration | Approximately 0.5mg/ml. Actual concentration varies with each lot. |
Application Info | WB: 1:1000 IHC-P: 1:50~100 IF: 1:10~50 FC: 1:10~50 |
Gene Symbol | PML |
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Alias Symbols | MYL, RNF71, PP8675, TRIM19 |
NCBI Gene Id | 5371 |
Protein Name | Protein PML |
Description of Target | PML is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). |
Uniprot ID | P29590 |
Protein Accession # | NP_002666.1 |
Molecular Weight | 97551 Da |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "PML Sumoylation Site Antibody (OAAB15905)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "PML Sumoylation Site Antibody (OAAB15905)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "PML Sumoylation Site Antibody (OAAB15905)" provided in?
This item is provided in "Liquid. PBS with 0.09% (W/V) sodium azide. ".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "PML Sumoylation Site Antibody (OAAB15905)"?
This target may also be called "MYL, RNF71, PP8675, TRIM19" in publications.
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What is the shipping cost for "PML Sumoylation Site Antibody (OAAB15905)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "PML Sumoylation Site Antibody (OAAB15905)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "PML Sumoylation Site Antibody (OAAB15905)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "97551 Da".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "PML Sumoylation Site Antibody (OAAB15905)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "PML"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "PML"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "PML"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "PML"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "PML"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "PML"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.