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Catalog No: OKCD00500 (Formerly GWB-KBBVL7)
Size:96WELLS
Price: $675.00
SKU
OKCD00500
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Datasheets/ManualsPrintable datasheet for PMFBP1 ELISA Kit (Human) (OKCD00500)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Polyamine Modulated Factor 1 Binding Protein 1 (PMFBP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Polyamine Modulated Factor 1 Binding Protein 1 (PMFBP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Polyamine Modulated Factor 1 Binding Protein 1 (PMFBP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Polyamine Modulated Factor 1 Binding Protein 1 (PMFBP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Polyamine Modulated Factor 1 Binding Protein 1 (PMFBP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Polyamine Modulated Factor 1 Binding Protein 1 (PMFBP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-PMFBP1 Microplate96 Wells (12 x 8 Well Strips)
PMFBP1 Lyophilized Standard2 x 10 ng
100X Biotinylated PMFBP1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.061 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Polyamine Modulated Factor 1 Binding Protein 1 (PMFBP1). No significant cross-reactivity or interference between Polyamine Modulated Factor 1 Binding Protein 1 (PMFBP1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolPMFBP1
Gene Full Namepolyamine modulated factor 1 binding protein 1
Alias SymbolsPMF-1 binding protein;polyamine-modulated factor 1-binding protein 1;SPGF31;STAP.
NCBI Gene Id83449
Protein NamePolyamine-modulated factor 1-binding protein 1
Description of TargetMay play a role in sperm morphology especially the sperm tail and consequently affect fertility. May also be involved in the general organization of cellular cytoskeleton.
Uniprot IDQ8TBY8
Protein Accession #NP_001153685
Nucleotide Accession #NM_001160213.1
Protein Size (# AA)1022
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