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Catalog No: OKEH06774
Size:96T
Price: $525.00
SKU
OKEH06774
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Pmel ELISA Kit (Mouse) (OKEH06774) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pmel has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Pmel is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pmel captured in the well.
ELISA Kit Range78-5000pg/ml
ELISA Kit RecoveryMean recovery when spiking into serum = 105%
ELISA Kit ReproducibilityIntra-Assay Reproducibility CV% < 5.9% (n=20)< br>Inter-Assay Reproducibility CV% < 9.3% (n=24)
ELISA Kit Component
ComponentAmount
IL3RA Microplate96 Wells (12 x 8 Well strips)
IL3RA Lyophilized Standard2 x 5 ng
100X Biotinylated IL3RA Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity25pg/ml
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPMEL
Gene Full Namepremelanosome protein
Alias SymbolsMelanocyte protein Pmel 17;Premelanosome protein;Silver locus protein.
NCBI Gene Id20431
Protein NameQ60696
Description of TargetMelanocyte protein PMEL
Uniprot IDhttps://www.uniprot.org/uniprot/Q60696
Protein Accession #NP_068682
Nucleotide Accession #NM_021882
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