Catalog No: OKEH04638
Size:96T
Price: $525.00
SKU
OKEH04638
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology PMCH ELISA Kit (Human) (OKEH04638) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PMCH has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for PMCH is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PMCH captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
Anti-PMCH Microplate96 Wells (12 x 8 Well Strips)
PMCH Lyophilized Standard2
Sample Diluent1 x 20 mL
PMCH Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.087 ng/mL
SpecificityNatural and recombinant Human Pro-MCH
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPMCH
Gene Full Namepro-melanin concentrating hormone
Alias SymbolsMCH;ppMCH;prepro-MCH;prepro-melanin-concentrating hormone;pro-MCH.
NCBI Gene Id5367
Protein NameP20382
Description of TargetPro-MCH
Uniprot IDhttps://www.uniprot.org/uniprot/P20382
Protein Accession #NP_002665.2
Nucleotide Accession #NM_002674.3
Protein Size (# AA)165
Molecular Weight19 KDa
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