PLUNC ELISA Kit (Human) (OKDD00474)

Catalog No: OKDD00474 (Formerly GWB-KBBFD3)

Size:96 Wells

Human PLUNC ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked immunosorbent assay
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~4 Hours
• ELISA Kit Principle: Aviva Systems Biology PLUNC ELISA Kit (Human) (OKDD00474) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for PLUNC has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PLUNC is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample PLUNC captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility:

  Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PLUNC were tested 20 times on one plate, respectively.

  Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PLUNC were tested on 3 different plates, 8 replicates in each plate.

  Intra-Assay: CV<10%

  Inter-Assay: CV<12%

• ELISA Kit Component:
  • Anti-PLUNC Microplate: 96 Wells (12 x 8 Well Strips)
  • PLUNC Lyophilized Standard: 2 x 10 ng
  • Standard Diluent: 1 x 20 mL
  • 100X PLUNC Biotinylated Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Detection Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Detection Conjugate Diluent: 1 x 12 mL
  • 10X Sample Diluent: 1 x 30 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: < 0.055 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of PLUNC. No significant cross-reactivity or interference between PLUNC and analogues was observed.
• Assay Info: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: BPIFA1
• Gene Full Name: BPI fold containing family A member 1
• Alias Symbols: bA49G10.5;BPI fold-containing family A member 1;ligand-binding protein RYA3;lung-specific protein X;LUNX;NASG;nasopharyngeal carcinoma-related protein;palate lung and nasal epithelium clone protein;palate, lung and nasal epithelium associated;PLUNC;protein Plunc;secretory protein in upper respiratory tracts;short PLUNC1;SPLUNC1;SPURT;tracheal epithelium enriched protein;Tracheal epithelium-enriched protein;von Ebner protein Hl.
• NCBI Gene Id: 51297
• Protein Name: BPI fold-containing family A member 1
• Description of Target: Plays a role in the innate immune responses of the upper airways. Reduces the surface tension in secretions from airway epithelia and inhibits the formation of biofilm by pathogenic Gram-negative bacteria, such as P.aeruginosa and K.pneumoniae. Binds bacterial lipopolysaccharide (LPS). Negatively regulates proteolytic cleavage of SCNN1G, an event that is required for activation of the epithelial sodium channel (ENaC), and thereby contributes to airway surface liquid homeostasis and proper clearance of mucus. Plays a role in the airway inflammatory response after exposure to irritants. May attract macrophages and neutrophils. May be associated with tumor progression.
• Uniprot ID: Q9NP55
• Protein Accession #: NP_001230122.1
• Nucleotide Accession #: NM_001243193.1
• Protein Size (# AA): 256
• Molecular Weight: 27 kDa