Catalog No: OKDD00473 (Formerly GWB-KBBES8)
Size:96 Wells
Price: $800.00
SKU
OKDD00473
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Datasheets/ManualsPrintable datasheet for PLOD1 ELISA Kit (Human) (OKDD00473)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked immunosorbent assay
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~4 Hours
ELISA Kit PrincipleAviva Systems Biology PLOD1 ELISA Kit (Human) (OKDD00473) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for PLOD1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PLOD1 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample PLOD1 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Reproducibility

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PLOD1 were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PLOD1 were tested on 3 different plates, 8 replicates in each plate.

Intra-Assay: CV<10%

Inter-Assay: CV<12%

ELISA Kit Component
ComponentAmount
Anti-PLOD1 Microplate96 Wells (12 x 8 Well Strips)
PLOD1 Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X PLOD1 Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell lysates
Sensitivity< 0.066 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of PLOD1. No significant cross-reactivity or interference between PLOD1 and analogues was observed.
Assay InfoQuantitative Sandwich ELISA
Gene SymbolPLOD1
Gene Full Nameprocollagen-lysine,2-oxoglutarate 5-dioxygenase 1
Alias SymbolsEDS6;EDSKCL1;LH;LH1;LLH;lysine hydroxylase;lysyl hydroxlase 1;Lysyl hydroxylase 1;PLOD;procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1;procollagen-lysine,2-oxoglutarate 5-dioxygenase 1.
NCBI Gene Id5351
Protein NameProcollagen-lysine,2-oxoglutarate 5-dioxygenase 1
Description of TargetPart of a complex composed of PLOD1, P3H3 and P3H4 that catalyzes hydroxylation of lysine residues in collagen alpha chains and is required for normal assembly and cross-linkling of collagen fibrils (By similarity). Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens (PubMed:8621606, PubMed:10686424, PubMed:15854030). These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links (Probable).
Uniprot IDQ02809
Protein Accession #NP_000293.2
Nucleotide Accession #NM_000302.3
Protein Size (# AA)727
Molecular Weight84 kDa
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