PLIN4 ELISA Kit (Human) (OKCD00929)

Catalog No: OKCD00929 (Formerly GWB-KBBDK3)

Size:96WELLS

Human PLIN4 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Perilipin 4 (PLIN4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Perilipin 4 (PLIN4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Perilipin 4 (PLIN4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Perilipin 4 (PLIN4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Perilipin 4 (PLIN4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Perilipin 4 (PLIN4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-PLIN4 Microplate: 96 Wells (12 x 8 Well Strips)
  • PLIN4 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated PLIN4 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.117 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Perilipin 4 (PLIN4). No significant cross-reactivity or interference between Perilipin 4 (PLIN4) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: PLIN4
• Gene Full Name: perilipin 4
• Alias Symbols: adipocyte protein S3-12;KIAA1881;perilipin-4;S3-12.
• NCBI Gene Id: 729359
• Protein Name: Perilipin-4
• Description of Target: May play a role in triacylglycerol packaging into adipocytes. May function as a coat protein involved in the biogenesis of lipid droplets (By similarity).
• Uniprot ID: Q96Q06
• Protein Accession #: NP_001073869
• Nucleotide Accession #: NM_001080400.1
• Protein Size (# AA): 1357