PKLR Antibody (OAGA00912)

Catalog No: OAGA00912

Size:100 ul

Sample (30 ug of whole cell lysate) A: 293T
B: A431
C: HeLa D: HepG2
E: A375
7.5% SDS PAGE
OAGA00912 diluted at 1:1000

Pyruvate Kinase (liver/RBC) antibody detects Pyruvate Kinase (liver/RBC) protein by Western blot analysis.
A. 20 ug Rat liver lysate/extract
7.5% SDS-PAGE
Pyruvate Kinase (liver/RBC) antibody (OAGA00912) dilution: 1:10000

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using Pyruvate Kinase (liver/RBC)(OAGA00912) antibody at 1:500 dilution.

Pyruvate Kinase (liver/RBC) antibody detects Pyruvate Kinase (liver/RBC) protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Pyruvate Kinase (liver/RBC) antibody (OAGA00912) diluted at 1:500.

Product Info

• Predicted Species Reactivity: Human, Rat
• Product Format: Liquid.0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7). 0.01% Thimerosal was added as a preservative.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Pyruvate Kinase (liver/RBC). The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Specificity: This antibody is raised against the R-Type isoform. Based on a BLAST result, it may also react with the L-Type isoform.
• Application Info: WB: 1:500-1:3000
  ICC/IF: 1:100-1:1000
  IHC-P: 1:100-1:1000
  Optimal dilutions should be determined by the end user.
• Publications:

  AG-348 enhances pyruvate kinase activity in red blood cells from patients with pyruvate kinase deficiency. Blood. 130, 1347-1356 (2017). 28760888

• Description:
  View product citations for PKLR Antibody (OAGA00912) on CiteAb

Target Info

• Gene Symbol: PKLR
• Gene Full Name: Pyruvate kinase PKLR
• Alias Symbols: PK1, PKL, RPK, PKRL
• NCBI Gene Id: 5313
• Protein Name: Pyruvate kinase isozymes R/L
• Description of Target: The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene.
• Uniprot ID: P30613
• Protein Accession #: NP_000289.1
• Molecular Weight: 62 kDa