PIWIL1 Antibody - N-terminal region (ARP41339_P050)

Catalog No: ARP41339_P050

WB Suggested Anti-PIWIL1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Human Stomach

WB Suggested Anti-PIWIL1 antibody Titration: 1 ug/mL
Sample Type: Human heart

Rabbit Anti-PIWIL1 Antibody
Catalog Number: ARP41339_P050
Formalin Fixed Paraffin Embedded Tissue: Human Adult heart
Observed Staining: Cytoplasmic
Primary Antibody Concentration: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy2/3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 – 2.0 sec
Protocol located in Reviews and Data.

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB, IHC
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PIWIL1
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 77%
• Peptide Sequence: Synthetic peptide located within the following region: FQELSLAERGGRRRDFHDLGVNTRQNLDHVKESKTGSSGIIVRLSTNHFR
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-PIWIL1 (ARP41339_P050) antibody is Catalog # AAP41339 (Previous Catalog # AAPP22694)
• Reference: Sugimoto,K., (2007) Biochem. Biophys. Res. Commun. 359 (3), 497-502
• Publications:

  Tex19 paralogs are new members of the piRNA pathway controlling retrotransposon suppression. J. Cell. Sci. 130, 1463-1474 (2017). 28254886

Target Info

• Gene Symbol: PIWIL1
• Gene Full Name: Piwi-like 1 (Drosophila)
• Alias Symbols: HIWI, MIWI, PIWI, CT80.1
• NCBI Gene Id: 9271
• Protein Name: Piwi-like protein 1
• Description of Target: PIWIL1 is a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. PI
• Uniprot ID: Q96J94
• Protein Accession #: NP_004755
• Nucleotide Accession #: NM_004764
• Protein Size (# AA): 861
• Molecular Weight: 98kDa
• Protein Interactions: UBC; DICER1; TP63; TP73; TP53;