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Catalog No: OKEH06793
Size:96T
Price: $625.00
SKU
OKEH06793
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Pip4k2c ELISA Kit (Rat) (OKEH06793) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pip4k2c has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Pip4k2c is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pip4k2c captured in the well.
ELISA Kit Range0.156-10ng/ml
ELISA Kit RecoveryMean recovery when spiking into serum = 89%
ELISA Kit ReproducibilityIntra-Assay Reproducibility CV% < 6.7% (n=20)< br>Inter-Assay Reproducibility CV% < 7.8% (n=24)
ELISA Kit Component
ComponentAmount
ADAM29 Microplate96 Wells (12 x 8 Well strips)
ADAM29 Lyophilized Standard2 x 10 ng
100X Biotinylated ADAM29 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.156 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPip4k2c
Gene Full Namephosphatidylinositol-5-phosphate 4-kinase type 2 gamma
Alias Symbolsphosphatidylinositol 5-phosphate 4-kinase type II gamma;phosphatidylinositol 5-phosphate 4-kinase type-2 gamma;phosphatidylinositol-4-phosphate 5-kinase, type II, gamma;PI(5)P 4-kinase type II gamma;PIP4KII-gamma;Pip5k2c.
NCBI Gene Id140607
Protein NameO88370
Description of TargetPhosphatidylinositol 5-phosphate 4-kinase type-2 gamma
Uniprot IDhttps://www.uniprot.org/uniprot/O88370
Protein Accession #NP_536728
Nucleotide Accession #NM_080480
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