PIK3CD ELISA Kit (Human) : 96 Wells (OKEH02353)

Catalog No: OKEH02353

Size:96T

Human PIK3CD ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology PIK3CD ELISA Kit (Human) (OKEH02353) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PIK3CD has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PIK3CD is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PIK3CD captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.4% (n = 20)
  Mean Inter-assay CV%: < 9.7% (n = 20)
• ELISA Kit Component:
  • PIK3CD Microplate: 96 Wells (12 x 8 Well strips)
  • PIK3CD Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated PIK3CA Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.089 ng/mL
• Specificity: Natural and recombinant Human Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: PIK3CD
• Gene Full Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta
• Alias Symbols: APDS;IMD14;IMD14A;IMD14B;p110D;P110DELTA;Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit delta;phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform;phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit delta;phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta short variant 20;phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta short variant 8;phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta short variant 8/20;phosphoinositide-3-kinase C;phosphoinositide-3-kinase, catalytic, delta polypeptide variant p37delta;PI3K;PI3Kdelta;PI3-kinase p110 subunit delta;ptdIns-3-kinase subunit p110-delta;ROCHIS.
• NCBI Gene Id: 5293
• Protein Name: O00329
• Description of Target: Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform
• Uniprot ID: https://www.uniprot.org/uniprot/O00329
• Protein Accession #: NP_005017.3
• Nucleotide Accession #: NM_005026.3
• Protein Size (# AA): 1044
• Molecular Weight: 119 kDa