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Datasheets/Manuals | Printable datasheet for PIK3C2A Antibody (OACD06489) |
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Predicted Species Reactivity | Human|Mouse|Rat |
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Product Format | Liquid. PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Application | Immunocytochemistry|Immunohistochemistry|Immunoprecipitation|Western blot |
Reconstitution and Storage | 2°C to 8°C|-20°C |
Immunogen | Range: Lys349 - Pro611 |
Purification | Affinity Purified; Protein A Purified |
Concentration | 0.5 mg/ml |
Preservative | 0.02% NaN3 |
Specificity | The antibody is a rabbit polyclonal antibody raised against PIK3C2a. It has been selected for its ability to recognize PIK3C2a in immunohistochemical staining and western blotting. |
Formulation | PBS, pH7.4, containing 0.02% NaN3, 50% glycerol |
Application Data | Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-1:5,000 Optimal working dilutions must be determined by end user. |
Gene Symbol | Pik3c2a |
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Gene Full Name | phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha |
Alias Symbols | Cpk-m;p170;phosphatidylinositol 3-kinase, C2 domain containing, alpha polypeptide;phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha;phosphoinositide 3-kinase-C2-alpha;PI3K;PI3KC2;PI3K-C2-alpha;ptdIns-3-kinase C2 subunit alpha. |
NCBI Gene Id | 18704 |
Protein Name | Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha |
Description of Target | Generates phosphatidylinositol 3-phosphate (PtdIns3P) and phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2) that act as second messengers. Has a role in several intracellular trafficking events. Functions in insulin signaling and secretion. Required for translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane and glucose uptake in response to insulin-mediated RHOQ activation. Regulates insulin secretion through two different mechanisms: involved in glucose-induced insulin secretion downstream of insulin receptor in a pathway that involves AKT1 activation and TBC1D4/AS160 phosphorylation, and participates in the late step of insulin granule exocytosis probably in insulin granule fusion. Synthesizes PtdIns3P in response to insulin signaling. Functions in clathrin-coated endocytic vesicle formation and distribution. Regulates dynamin-independent endocytosis, probably by recruiting EEA1 to internalizing vesicles. In neurosecretory cells synthesizes PtdIns3P on large dense core vesicles. Participates in calcium induced contraction of vascular smooth muscle by regulating myosin light chain (MLC) phosphorylation through a mechanism involving Rho kinase-dependent phosphorylation of the MLCP-regulatory subunit MYPT1. May play a role in the EGF signaling cascade. May be involved in mitosis and UV-induced damage response. Required for maintenance of normal renal structure and function by supporting normal podocyte function. |
Uniprot ID | Q61194 |
Protein Accession # | NP_035213.2 |
Nucleotide Accession # | NM_011083 |
Molecular Weight | 188 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "PIK3C2A Antibody (OACD06489)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "PIK3C2A Antibody (OACD06489)"?
This item is available "Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery".
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What buffer format is "PIK3C2A Antibody (OACD06489)" provided in?
This item is provided in "Liquid. PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "PIK3C2A Antibody (OACD06489)"?
This target may also be called "Cpk-m;p170;phosphatidylinositol 3-kinase, C2 domain containing, alpha polypeptide;phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha;phosphoinositide 3-kinase-C2-alpha;PI3K;PI3KC2;PI3K-C2-alpha;ptdIns-3-kinase C2 subunit alpha." in publications.
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What is the shipping cost for "PIK3C2A Antibody (OACD06489)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "PIK3C2A Antibody (OACD06489)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "PIK3C2A Antibody (OACD06489)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "188 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "PIK3C2A Antibody (OACD06489)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "Pik3c2a"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "Pik3c2a"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "Pik3c2a"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "Pik3c2a"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "Pik3c2a"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "Pik3c2a"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.