PIK3C2A Antibody - C-terminal region (OAGA02342)

Catalog No: OAGA02342

Size:100 ul

Sample (30 ug of whole cell lysate)
A: A431
5% SDS PAGE
OAGA02342 diluted at 1:1000

PI3K C2 alpha antibody [C3], C-term detects PIK3C2A protein at cytosol on hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
PI3K C2 alpha antibody [C3], C-term (OAGA02342) dilution: 1:200.

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using PI3K C2 alpha (OAGA02342) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled (Red) at 1:500.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IF, IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PI3K C2 alpha. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.8mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Target Info

• Gene Symbol: PIK3C2A
• Gene Full Name: phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 alpha
• Alias Symbols: CPK, OCSKD, PI3-K-C2A, PI3K-C2alpha, PI3K-C2-alpha, PI3-K-C2(ALPHA)
• NCBI Gene Id: 5286
• Description of Target: The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is not sensitive to nanomolar levels of the inhibitor wortmanin. This protein was shown to be able to be activated by insulin and may be involved in integrin-dependent signaling.
• Uniprot ID: O00443