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Datasheets/Manuals | Printable datasheet for OAAB08098 |
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Predicted Species Reactivity | Mouse |
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Product Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Clonality | Polyclonal |
Isotype | Ig |
Host | Rabbit |
Application | WB, IHC |
Additional Information | Precautions: PIK3C2A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
:: | Function: Generates phosphatidylinositol 3-phosphate (PtdIns3P) and phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2) that act as second messengers. Has a role in several intracellular trafficking events. Functions in insulin signaling and secretion Required for translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane and glucose uptake in response to insulin- mediated RHOQ activation. Regulates insulin secretion through two different mechanisms: involved in glucose-induced insulin secretion downstream of insulin receptor in a pathway that involves AKT1 activation and TBC1D4/AS160 phosphorylation, and participates in the late step of insulin granule exocytosis probably in insulin granule fusion. Synthesizes PtdIns3P in response to insulin signaling. Functions in clathrin-coated endocytic vesicle formation and distribution. Regulates dynamin- independent endocytosis, probably by recruiting EEA1 to internalizing vesicles. In neurosecretory cells synthesizes PtdIns3P on large dense core vesicles. Participates in calcium induced contraction of vascular smooth muscle by regulating myosin light chain (MLC) phosphorylation through a mechanism involving Rho kinase-dependent phosphorylation of the MLCP-regulatory subunit MYPT1. May play a role in the EGF signaling cascade. May be involved in mitosis and UV-induced damage response. Required for maintenance of normal renal structure and function by supporting normal podocyte function |
:: | Cellular Location: Cell membrane. Golgi apparatus. Cytoplasmic vesicle, clathrin-coated vesicle. Nucleus. Cytoplasm Note=According to PubMed:10766823 and PubMed:11239472, it is found in the cell membrane, the Golgi apparatus and in clathrin-coated vesicles. According to PubMed:17038310 it inserts preferentially into membranes containing PtdIns(4,5)P2. According to PubMed:11606566, it is nuclear and cytoplasmic. Asso Tissue Location: Expressed in columnar and transitional epithelia, mononuclear cells, smooth muscle cells, and endothelial cells lining capillaries and small venules (at protein level) Ubiquitously expressed, with highest levels in heart, placenta and ovary, and lowest levels in the kidney. Detected at low levels in islets of Langerhans from type 2 diabetes mellitus individuals |
Reconstitution and Storage | Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
Immunogen | This PIK3C2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1431-1458 amino acids from the C-terminal region of human PIK3C2A. |
Predicted Homology Based on Immunogen Sequence | Mouse |
Concentration | Approximately 0.5mg/ml. Actual concentration varies with each lot. |
Application Info | WB: 1:1000 IHC-P: 1:10~50 |
Gene Symbol | PIK3C2A |
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Alias Symbols | CPK, OCSKD, PI3-K-C2A, PI3K-C2alpha, PI3K-C2-alpha, PI3-K-C2(ALPHA) |
NCBI Gene Id | 5286 |
Protein Name | Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha |
Description of Target | Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Uniprot ID | O00443 |
Protein Accession # | NP_002636.2 |
Molecular Weight | 190680 Da |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "PIK3C2A Antibody - C-terminal region (OAAB08098)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Mouse".
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How long will it take to receive "PIK3C2A Antibody - C-terminal region (OAAB08098)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "PIK3C2A Antibody - C-terminal region (OAAB08098)" provided in?
This item is provided in "Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "PIK3C2A Antibody - C-terminal region (OAAB08098)"?
This target may also be called "CPK, OCSKD, PI3-K-C2A, PI3K-C2alpha, PI3K-C2-alpha, PI3-K-C2(ALPHA)" in publications.
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What is the shipping cost for "PIK3C2A Antibody - C-terminal region (OAAB08098)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "PIK3C2A Antibody - C-terminal region (OAAB08098)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "PIK3C2A Antibody - C-terminal region (OAAB08098)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "190680 Da".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "PIK3C2A Antibody - C-terminal region (OAAB08098)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "PIK3C2A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "PIK3C2A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "PIK3C2A"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "PIK3C2A"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "PIK3C2A"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "PIK3C2A"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.