PICALM Antibody (OAPB01019)

Catalog No: OAPB01019

Size:100UG

Immunofluorescence of PICALM in human brain tissue with PICALM antibody at 20 μg/mL.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. PICALM Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Western blot
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: PICALM antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: PICALM antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human PICALM. The immunogen is located within the first 50 amino acids of PICALM.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-PICALM antibody is Catalog # OEPB03134
• Application Info: PICALM antibody can be used for detection of PICALM by Western blot at 1 - 2 ug/ml. (Optimal dilution should be determined by user)
• Reference: Tebar F, Bohlander SK, and Sorkin A. Clathrin assembly lymphoid myeloid leukemia (CALM) protein: localization in endocytic-coated pits, interactions with clathrin, and the impact of overexpression on clathrin mediated traffic. Mol. Biol. Cell1999; 10:2687-702.;Caudell D and Aplan PD. The role of CALM-AF10 gene fusion in acute leukemia. Leukemia2008; 22:678-85.;Harel A, Wu F, Mattson MP, et al. Evidence for CALM in directing VAMP2 trafficking. Traffic2008; 9:417-29.;Harold D, Abraham R, Hollingworth P, et al. Genome-wide association study identifies variants at CLU and PICALM associated with Alzheimer’s disease. Nat. Genet.2009; 41:1088-93.

Target Info

• Gene Symbol: PICALM
• Gene Full Name: phosphatidylinositol binding clathrin assembly protein
• Alias Symbols: CALM;clathrin assembly lymphoid myeloid leukemia protein;CLTH;LAP;phosphatidylinositol-binding clathrin assembly protein.
• NCBI Gene Id: 8301
• Protein Name: Phosphatidylinositol-binding clathrin assembly protein|phosphatidylinositol-binding clathrin assembly protein isoform 1 [Homo sapiens]
• Description of Target: Assembly protein recruiting clathrin and adapter protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction.
• Uniprot ID: Q13492
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_009097