PIAS1 Antibody - N-terminal region (ARP39231_P050)

Catalog No: ARP39231_P050

WB Suggested Anti-PIAS1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Human Placenta

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PIAS1
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%
• Peptide Sequence: Synthetic peptide located within the following region: MADSAELKQMVMSLRVSELQVLLGYAGRNKHGRKHELLTKALHLLKAGCS
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-PIAS1 (ARP39231_P050) antibody is Catalog # AAP39231 (Previous Catalog # AAPY00843)
• Reference: Helbig,K.J., (2008) Antiviral Res. 77 (3), 169-176

Target Info

• Gene Symbol: PIAS1
• Gene Full Name: Protein inhibitor of activated STAT, 1
• Alias Symbols: GBP, ZMIZ3, DDXBP1, GU/RH-II
• NCBI Gene Id: 8554
• Protein Name: E3 SUMO-protein ligase PIAS1
• Description of Target: PIAS1 is a member of the mammalian PIAS [protein inhibitor of activated STAT-1 (signal transducer and activator of transcription-1)] family. This member contains a putative zinc-binding motif and a highly acidic region. It inhibits STAT1-mediated gene activation and the DNA binding activity, binds to Gu protein/RNA helicase II/DEAD box polypeptide 21, and interacts with androgen receptor (AR). It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis.This gene encodes a member of the mammalian PIAS [protein inhibitor of activated STAT-1 (signal transducer and activator of transcription-1)] family. This member contains a putative zinc-binding motif and a highly acidic region. It inhibits STAT1-mediated gene activation and the DNA binding activity, binds to Gu protein/RNA helicase II/DEAD box polypeptide 21, and interacts with androgen receptor (AR). It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Uniprot ID: O75925
• Protein Accession #: NP_057250
• Nucleotide Accession #: NM_016166
• Protein Size (# AA): 651
• Molecular Weight: 72kDa
• Protein Interactions: STAT1; TRIM63; TRIM55; CEP250; PRDM1; SUMO1; L3MBTL2; CBS; SHFM1; SUMO2; SUMO3; SOX2; CHUK; SPOP; YWHAZ; UBC; SKIL; SGTA; ATXN1; SMAD7; SMAD4; SMAD1; HTT; PRPF40A; PPP1CC; BARD1; PAXIP1; NR1H2; PPARGC1A; NR1H3; PPARGC1B; RAD54L2; ZMYND11; UBD; PLAG1; MYB;