PI4KA ELISA Kit (Human) (OKCD01280)

Catalog No: OKCD01280 (Formerly GWB-KBBDG8)

Size:96WELLS

Human PI4KA ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphatidylinositol-4-Kinase Catalytic Alpha (PI4Ka). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphatidylinositol-4-Kinase Catalytic Alpha (PI4Ka). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphatidylinositol-4-Kinase Catalytic Alpha (PI4Ka), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Phosphatidylinositol-4-Kinase Catalytic Alpha (PI4Ka) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phosphatidylinositol-4-Kinase Catalytic Alpha (PI4Ka) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phosphatidylinositol-4-Kinase Catalytic Alpha (PI4Ka) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-PI4KA Microplate: 96 Wells (12 x 8 Well Strips)
  • PI4KA Lyophilized Standard: 2 x 50 ng
  • 100X Biotinylated PI4KA Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.27 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Phosphatidylinositol-4-Kinase Catalytic Alpha (PI4Ka). No significant cross-reactivity or interference between Phosphatidylinositol-4-Kinase Catalytic Alpha (PI4Ka) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: PI4KA
• Gene Full Name: phosphatidylinositol 4-kinase alpha
• Alias Symbols: phosphatidylinositol 4-kinase 230;phosphatidylinositol 4-kinase alpha;phosphatidylinositol 4-kinase III alpha;phosphatidylinositol 4-kinase IIII+/-;phosphatidylinositol 4-kinase, catalytic, alpha;phosphatidylinositol 4-kinase, type III, alpha;pi4K230;PI4K-ALPHA;PI4-kinase alpha;PIK4CA;PMGYCHA;ptdIns-4-kinase alpha;testicular secretory protein Li 35.
• NCBI Gene Id: 5297
• Protein Name: Phosphatidylinositol 4-kinase alpha
• Description of Target: Acts on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate.
• Uniprot ID: P42356
• Protein Accession #: NP_477352
• Nucleotide Accession #: NM_058004.3
• Protein Size (# AA): 2102