PHOX2B Antibody - middle region (OAAB07587)

Catalog No: OAAB07587

Size:400ul

PHOX2B Antibody (Center) (Cat. #OAAB07587) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the PHOX2B antibody detected the PHOX2B protein (arrow).

Fluorescent image of U251 cell stained with PHOX2B Antibody (Center)(Cat#OAAB07587).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PHOX2B primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C).PHOX2B immunoreactivity is localized to Nucleus significantly.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IF
• Additional Information: Precautions: PHOX2B Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Involved in the development of several major noradrenergic neuron populations, including the locus coeruleus Transcription factor which could determine a neurotransmitter phenotype in vertebrates. Enhances second-messenger-mediated activation of the dopamine beta-hydrolase and c-fos promoters, and of several enhancers including cAMP-response element and serum- response element
  Cellular Location: Nucleus (By similarity).
  Tissue Location: Expressed in neuroblastoma, brain and adrenal gland
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This PHOX2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-130 amino acids from the Central region of human PHOX2B.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: IF: 1:10~50
  WB: 1:1000

Target Info

• Gene Symbol: PHOX2B
• Alias Symbols: CCHS, PMX2B, NBLST2, NBPhox
• NCBI Gene Id: 8929
• Protein Name: Paired mesoderm homeobox protein 2B
• Description of Target: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q99453
• Protein Accession #: NP_003915.2
• Molecular Weight: 31621 Da