PHF8 ELISA Kit (Mouse) (OKCD01031)

Catalog No: OKCD01031 (Formerly GWB-KBBMP9)

Size:96WELLS

Mouse Phf8 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PHD Finger Protein 8 (PHF8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to PHD Finger Protein 8 (PHF8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PHD Finger Protein 8 (PHF8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PHD Finger Protein 8 (PHF8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PHD Finger Protein 8 (PHF8) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PHD Finger Protein 8 (PHF8) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Phf8 Microplate: 96 Wells (12 x 8 Well Strips)
  • Phf8 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated Phf8 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.118 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of PHD Finger Protein 8 (PHF8). No significant cross-reactivity or interference between PHD Finger Protein 8 (PHF8) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Phf8|Phf8-ps
• Gene Full Name: PHD finger protein 8|PHD finger protein 8, pseudogene
• Alias Symbols: [histone H3]-dimethyl-L-lysine(36) demethylase PHF8;[histone H3]-dimethyl-L-lysine(9) demethylase PHF8;4921501E09Rik;9830141C09Rik;histone lysine demethylase PHF8;KIAA1111-like protein;mKIAA1111;PHD finger protein 8;PHF8.
• NCBI Gene Id: 320595|74042
• Protein Name: Histone lysine demethylase PHF8
• Description of Target: Histone lysine demethylase with selectivity for the di- and monomethyl states that plays a key role cell cycle progression, rDNA transcription and brain development. Demethylates mono- and dimethylated histone H3 'Lys-9' residue (H3K9Me1 and H3K9Me2), dimethylated H3 'Lys-27' (H3K27Me2) and monomethylated histone H4 'Lys-20' residue (H4K20Me1). Acts as a transcription activator as H3K9Me1, H3K9Me2, H3K27Me2 and H4K20Me1 are epigenetic repressive marks. Involved in cell cycle progression by being required to control G1-S transition. Acts as a coactivator of rDNA transcription, by activating polymerase I (pol I) mediated transcription of rRNA genes. Required for brain development, probably by regulating expression of neuron-specific genes. Has activity toward H4K20Me1 only when nucleosome is used as a substrate and when not histone octamer is used as substrate. May also have weak activity toward dimethylated H3 'Lys-36' (H3K36Me2), however, the relevance of this result remains unsure in vivo. Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: has weak activity toward H3K9Me2 in absence of H3K4me3, while it has high activity toward H3K9me2 when binding H3K4me3.
• Uniprot ID: Q80TJ7
• Protein Accession #: NP_001009544
• Nucleotide Accession #: NM_001009544.2
• Protein Size (# AA): 1023