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Datasheets/Manuals | Printable datasheet for anti-PGP9.5 antibody - OAPA00292 |
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Predicted Species Reactivity | Human |
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Product Format | 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide. |
Clonality | Monoclonal |
Clone | P3D9AT. |
Isotype | Mouse IgG2a heavy chain and kappa light chain. |
Host | Mouse |
Application | WB, IHC |
:: | Product Type: Mouse Anti Human Monoclonal. |
:: | Product Introduction: UCHL1 (PGP9.5) belongs to a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to produce the ubiquitin monomer. Protein Gene Product (PGP9.5) is a neuron specific protein, structurally and immunologically distinct from neuron specific enolase. Human UCHL1 and UCHL3 have an extremely complicated knot structure for a protein, with five knot crossings. It is considered that the knot structure may increase a protein''s resistance to degradation in the proteasome. The protein, which has a molecular weight of 27 kDa was first defined by high resolution two dimetional PAGE. Standard immunohistochemical techniques have demonstrated the presence of PGP9.5 in neurons and nerve fibers at all levels of the central and peripheral nervous system, in many neurodcrine cells, in segments of the renal tubules, in spermatogonia and leydig cells of the testis, in ova and in some cells of both the pregnant and non pregnant corpus luteum. A point mutation (I93M) in UCHL1 is implicat |
Reconstitution and Storage | For periods up to 1 month store at 4C, for longer periods of time, store at -20C. Prevent freeze thaw cycles. Stability: 12 months at -20C. 1 month at 4C. |
Immunogen | The immunogen for anti-PGP9.5 antibody: anti-human PGP9.5 mAb is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with recombinant human PGP9.5 amino acids 1-223 purified from E. coli. |
Purity | PGP9.5 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography. |
Application Info | PGP9.5 antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:500 ~ 1:2,000 and immunohistochemistry analysis is 1:100~200. Recommended starting dilution for Western blot is 1:1,000 and Immunohistochemistry is 1:100. |
Gene Symbol | PGP9.5 |
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Alias Symbols | NDGOA, PARK5, PGP95, SPG79, PGP9.5, Uch-L1, HEL-117, PGP 9.5, HEL-S-53 |
NCBI Gene Id | 7345 |
Protein Name | Ubiquitin carboxyl-terminal hydrolase isozyme L1 |
Description of Target | Mouse Anti Human PGP9.5 |
Uniprot ID | P09936 |
Protein Accession # | NP_004172.2 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "PGP9.5 Antibody (OAPA00292)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "PGP9.5 Antibody (OAPA00292)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "PGP9.5 Antibody (OAPA00292)" provided in?
This item is provided in "1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "PGP9.5 Antibody (OAPA00292)"?
This target may also be called "NDGOA, PARK5, PGP95, SPG79, PGP9.5, Uch-L1, HEL-117, PGP 9.5, HEL-S-53" in publications.
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What is the shipping cost for "PGP9.5 Antibody (OAPA00292)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "PGP9.5 Antibody (OAPA00292)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "PGP9.5 Antibody (OAPA00292)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "PGP9.5 Antibody (OAPA00292)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "PGP9.5"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "PGP9.5"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "PGP9.5"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "PGP9.5"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "PGP9.5"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "PGP9.5"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.