PGLYRP1 ELISA Kit (Mouse) (OKCD00197)

Catalog No: OKCD00197 (Formerly GWB-KBBHO7)

Size:96WELLS

Mouse Pglyrp1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	80-102%	83-105%	96-105%	97-105%
  EDTA plasma(n=5)	82-104%	90-98%	94-103%	86-101%
  heparin plasma(n=5)	97-104%	98-105%	96-104%	81-98%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peptidoglycan Recognition Protein 1 (PGLYRP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peptidoglycan Recognition Protein 1 (PGLYRP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peptidoglycan Recognition Protein 1 (PGLYRP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Peptidoglycan Recognition Protein 1 (PGLYRP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	98-105	102
  EDTA plasma(n=5)	85-94	89
  heparin plasma(n=5)	78-96	88

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peptidoglycan Recognition Protein 1 (PGLYRP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peptidoglycan Recognition Protein 1 (PGLYRP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Pglyrp1 Microplate: 96 Wells (12 x 8 Well Strips)
  • Pglyrp1 Lyophilized Standard: 2 x 1 ng
  • 100X Biotinylated Pglyrp1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: < 5.9 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Peptidoglycan Recognition Protein 1 (PGLYRP1). No significant cross-reactivity or interference between Peptidoglycan Recognition Protein 1 (PGLYRP1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Pglyrp1
• Gene Full Name: peptidoglycan recognition protein 1
• Alias Symbols: cytokine tag7;peptidoglycan recognition protein 1;Peptidoglycan recognition protein short;PG;Pglyrp;PGRP;PGRP-S;Tag;Tag7;Tas;Tasg7;Tnfs;Tnfsf3l.
• NCBI Gene Id: 21946
• Protein Name: Peptidoglycan recognition protein 1
• Description of Target: Pattern receptor that binds to murein peptidoglycans (PGN) of Gram-positive bacteria. Has bactericidal activity towards Gram-positive bacteria. May kill Gram-positive bacteria by interfering with peptidoglycan biosynthesis. Binds also to Gram-negative bacteria. Involved in innate immunity. May function in intracellular killing of bacteria. The soluble form triggers apoptosis in vitro.
• Uniprot ID: O88593
• Protein Accession #: NP_033428
• Nucleotide Accession #: NM_009402.2
• Protein Size (# AA): 182