PGC ELISA Kit (Mouse) (OKCD00225)

Catalog No: OKCD00225 (Formerly GWB-KBBOY6)

Size:96WELLS

Mouse Pgc ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	83-93%	90-104%	98-105%	94-101%
  EDTA plasma(n=5)	81-94%	85-97%	80-104%	80-88%
  heparin plasma(n=5)	90-98%	85-96%	94-102%	90-97%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pepsinogen C (PGC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pepsinogen C (PGC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pepsinogen C (PGC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Pepsinogen C (PGC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	95-102	99
  EDTA plasma(n=5)	80-91	86
  heparin plasma(n=5)	91-101	95

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pepsinogen C (PGC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pepsinogen C (PGC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Pgc Microplate: 96 Wells (12 x 8 Well Strips)
  • Pgc Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated Pgc Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: < 0.063 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Pepsinogen C (PGC). No significant cross-reactivity or interference between Pepsinogen C (PGC) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Pgc
• Gene Full Name: progastricsin (pepsinogen C)
• Alias Symbols: 2210410L06Rik;gastricsin;pepsinogen C;Upg;Upg-;Upg1;Upg-1;urinary pepsinogen 1.
• NCBI Gene Id: 109820
• Protein Name: Gastricsin
• Description of Target: Hydrolyzes a variety of proteins.
• Uniprot ID: Q9D7R7
• Protein Accession #: NP_080249
• Nucleotide Accession #: NM_025973.3
• Protein Size (# AA): 392