PGA5 ELISA Kit (Human) (OKCD01108)

Catalog No: OKCD01108 (Formerly GWB-KBBNB8)

Size:96WELLS

Human PGA5 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	90-103%	88-102%	95-104%	94-101%
  EDTA plasma(n=5)	82-93%	85-99%	80-102%	82-101%
  heparin plasma(n=5)	98-105%	86-95%	81-96%	94-102%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pepsinogen A (PGA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pepsinogen A (PGA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pepsinogen A (PGA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Pepsinogen A (PGA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 3.12-200ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	86-101	92
  EDTA plasma(n=5)	96-104	101
  heparin plasma(n=5)	99-105	102

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pepsinogen A (PGA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pepsinogen A (PGA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-PGA5 Microplate: 96 Wells (12 x 8 Well Strips)
  • PGA5 Lyophilized Standard: 2 x 200 ng
  • 100X Biotinylated PGA5 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 1.27 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Pepsinogen A (PGA). No significant cross-reactivity or interference between Pepsinogen A (PGA) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: PGA3|PGA4|PGA5
• Gene Full Name: pepsinogen A3|pepsinogen A4|pepsinogen A5
• Alias Symbols: pepsin A;pepsin A-3;pepsin A-4;pepsin A-5;pepsinogen 3, group I (pepsinogen A);pepsinogen 4, group I (pepsinogen A);pepsinogen 5, group I (pepsinogen A);pepsinogen-3;pepsinogen-4;pepsinogen-5;Pg5;pregnancy-associated glycoprotein.
• NCBI Gene Id: 5222|643834|643847
• Protein Name: Pepsin A-3|Pepsin A-4|Pepsin A-5
• Description of Target: Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.
• Uniprot ID: P00790
• Protein Accession #: NP_055039
• Nucleotide Accession #: NM_014224.4
• Protein Size (# AA): 388