Catalog No: OKEH00389
Size:96T
Price: $525.00
SKU
OKEH00389
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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PFN2 ELISA Kit (Human) : 96 Wells (OKEH00389)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PFN2 ELISA Kit (Human) (OKEH00389) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human PFN2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated PFN2 and incubated. The PFN2 found in the sample or standards competes with the biotinylated PFN2 for limited binding sites on the immobilized anti-human PFN2 antibody. Excess unbound biotinylated PFN2 and sample or standard PFN2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated PFN2 captured in the well and inversely proportional to the amount of PFN2 which was contained in the sample or standard.
ELISA Kit Range0.625-40ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.8% (n = 20)
Mean Inter-assay CV%: < 8.2% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-PFN2 Microplate96 Wells (12 x 8 Well strips)
PFN2 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X PFN2-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.33 ng/mL
SpecificityNatural and recombinant Human Profilin-2
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolPFN2
Gene Full Nameprofilin 2
Alias SymbolsD3S1319E;PFL;profilin II;profilin-2.
NCBI Gene Id5217
Protein NameP35080
Description of TargetProfilin-2
Uniprot IDhttps://www.uniprot.org/uniprot/P35080
Protein Accession #NP_002619.1
Nucleotide Accession #NM_002628.4
Protein Size (# AA)140
Molecular Weight15 kDa
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